Chemistry: molecular biology and microbiology – Carrier-bound or immobilized enzyme or microbial cell;...
Reexamination Certificate
2006-05-30
2006-05-30
Witz, Jean C. (Department: 1651)
Chemistry: molecular biology and microbiology
Carrier-bound or immobilized enzyme or microbial cell;...
C435S176000, C435S180000, C435S181000
Reexamination Certificate
active
07052887
ABSTRACT:
The invention relates to a method of immobilizing an affinity reagent or affinity reagents on a hydrophobic solid phase which can be used in biological assays for analyte detection. The invention relates more particularly to a method of immobilizing an affinity reagent on a hydrophobic solid phase functionalized by a carboxyl group, said method comprising a step for activation of said solid phase and a step for coupling of the affinity reagent to said solid phase, characterized in that the step for activation of said solid phase uses a combination of a carbodiimide and a phosphate buffer in the presence of a co-activator and in an acid medium, and in that the coupling step is performed in a basic medium.The invention further relates to the reactive complexes obtained by this method and to their use in immunoassay kits, hybridization kits or enzymatic assay kits.
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Babin Fabienne
Hamon Laurence
Rieunier François
Bio-Rad Pasteur
Hanley Susan
Merchant & Gould P.C.
Witz Jean C.
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