Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1995-11-29
1997-10-07
Sisson, Bradley L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 911, 435 912, 435270, 536 231, 536 2433, 536 753, 935 76, 935 77, 935 78, C12Q 168, C12P 1934, C07H 2104, C12N 1500
Patent
active
056746873
ABSTRACT:
Microsatellite nucleotide repeat sequences present in DNA isolated from a biological sample are used to determine the origin of a biological sample by a process including the steps of isolating nucleic acid from a biological sample, determining the interspersion pattern of repeats of a nucleotide sequence selected from the group consisting of di-, tri-, and tetra- microsatellite nucleotide repeat sequences in the DNA, and comparing the determined interspersion pattern with known interspersion patterns of the nucleotide sequence in selected mammalian species.
Species-specific, individually-invariant interspersion patterns of di-, tri-, or tetra- microsatellite nucleotide repeats in vertebrate DNA samples are identified by a process including the step of annealing separated strands with a oligonucleotide primer selected from the group consisting of di-, tri- and tetra- microsatellite nucleotide repeat sequences at a concentration ratio of primer to DNA template of less than about 2500:1, the annealing being conducted at a temperature greater than about 30.degree. C. and in the presence of magnesium ions at a concentration less than about 3.0 mM, to produce an extension product of the primer which is complementary to the strand of the DNA sample.
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Cornell Research Foundation Inc.
Sisson Bradley L.
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