Method for identifying the elution time of an analyte

Details Method for identifying the elution time of an analyte Method for identifying the elution time of an analyte

2011-03-01

2011-03-01

Griffin, Walter D (Department: 1774)

Chemistry: analytical and immunological testing

Including chromatography

C436S086000, C702S019000, C702S001000

Reexamination Certificate

active

07897405

ABSTRACT:
A method for determining a time of elution of a peptide of interest from a liquid chromatography column includes a step of obtaining chromatographic data for each of a plurality of candidate fragment ions of the peptide of interest. A time along a common chromatographic time is scale determined corresponding to maximum overlay of the ion signals measured for each of the plurality of candidate fragment ions. Finally, the determined time is assigned as the time of elution of the peptide of interest from the liquid chromatography column. In particular, the chromatographic data is acquired during selective reaction monitoring of an eluate from the liquid chromatography column containing the peptide of interest. The chromatographic data includes ion signals measured along the common chromatographic time scale for each of the plurality of candidate fragment ions.

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