Method for identifying target bacteria

Details Method for identifying target bacteria Method for identifying target bacteria

1996-07-12

1999-03-30

Gitomer, Ralph

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 34, 435 39, C12Q 170

Patent

active

058887253

ABSTRACT:
A method for detection, identification and/or quantification of target organisms of specific bacterial genus, species or serotype, based upon the occurence of release of cell contents, particularly nucleotides, e.g., ATP, on lysis of bacterial cell walls on incubation with bacteriophages (phages) specific for them. When new phase particles are released at the end of the phage replication cycle nucleotide levels are measured and compared with controls. The method provides for the detection of specific bacteria which does not require insertion of the lux gene into the phage genome yet is faster and more sensitive than known non-modified phage utilizing techniques. The method is only limited by the availability of phage types suitable for selective attack of the target bacterial to be detected and can detect a single Salmonella in a sample of milk in under 12 hours.

REFERENCES:
patent: 4104126 (1978-08-01), Young
patent: 4861709 (1989-08-01), Ulitzur et al.
patent: 5085982 (1992-02-01), Keith
Hirsh, D., Rapid Detection of Salmonella in Certified Raw Milk by Using Charge Modified Filters and Felix-01 Bacteriophage, J of Food Protection, 47(5):388-390, May 1984.

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