Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase
Reexamination Certificate
2006-07-11
2006-07-11
Lankford, Jr., Leon B. (Department: 1651)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving hydrolase
C435S068100, C435S195000, C356S344000, C204S450000
Reexamination Certificate
active
07074583
ABSTRACT:
A method for identifying phosphoprotein, which comprises: subjecting a sample protein to two-dimensional electrophoresis; dephosphorylating the sample protein with phosphatase; once again performing the two-dimensional electrophoresis under the same conditions; and detecting the spot that migrates to the alkaline side of the isoelectric focusing as the phosphoprotein is provided as a convenient method for identifying phosphoprotein with high accuracy.
REFERENCES:
Robert W. Leggett et al., The Journal of Biological Chemistry, Oct. 1995, vol. 270, No. 43, p. 25882.
M. Butler et al., Journal of Neurochemistry, 1986, vol. 47, No. 5, pp. 1520, 1521.
Kristensen Dan Bach
Yoshizato Katsutoshi
Hiroshima Industrial Promotion Organization
Japan Science and Technology Corporation
Lankford , Jr. Leon B.
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