Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1988-12-09
1991-08-20
Teskin, Robin L.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
4351721, 4352402, 435 721, 435 76, 935 50, 935 76, C12N 1500, C12N 500, G01N 3353
Patent
active
050413767
ABSTRACT:
The present invention relates to a method for identifying or shielding functional sites or epitopes of proteins that enter the exocytotic pathway of eukaryotic cells (transportable proteins) by the addition of supernumerary N-linked oligosaccharide side chains at chosen sites on the surface thereof using oligonucleotide mutagenesis. The present invention also relates to mutant transportable proteins having supernumerary N-linked oligosaccharide side chains which shield functional sites or epitopes; and genes which encode the same.
REFERENCES:
Guan et al. (1985), "Glycosylation Allows Cell-Surface Transport of a Secretory Protein", Cell, vol. 42, pp. 489-496.
Dixon et al. (1987), "Structural Features for Ligand Binding to .beta.-ar", Embo J., vol. 6, pp. 3269-3275.
Skehel et al. (1984), "A Carbohydrate Side Chain on Hemoglutinins . . . Inhibits Recognition by Antibody", PNAS, vol. 81, pp. 1779-1783.
1984 Cold Spring Harbor Annual Report (Aug. 1985), pp. 65-77.
J. Cell. Biochem., Supp. 11A, p. 274, Abstract E215.
J. Cell. Biochem., Supp. 11A, p. 248, Abstract E019.
Gallagher, P. et al., J. Cell. Biol., 107:2059-2073 (1988).
Gallagher Patricia
Gething Mary J.
Sambrook Joseph F.
Cold Spring Harbor Laboratory
Lebovitz Richard
Teskin Robin L.
The Board of Regents of the University of Texas System
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