Method for identifying mutagenic agents which induce large, mult

Details Method for identifying mutagenic agents which induce large, mult Method for identifying mutagenic agents which induce large, mult

1990-05-17

1993-07-13

Wax, Robert A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 29, 435156, 4351721, 4352401, 935 10, 935 34, C12G 168, C12G 102, C12N 1500, C12N 500

Patent

active

052272893

ABSTRACT:
An assay is disclosed for identifying mutagenic agents which include large, multilocus deletion events in cultured mammalian cells. The cell lines used are adenine phosphoribosyltransferase heterozygotes derived from CHO cells, which we disclose possess a genomic sequence variation near the above-mentioned gene. We present the nucleotide sequence surrounding this variation, enabling an efficient determination of the proportion of deletion mutants among the mutant colonies induced by the agent to be tested.

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