Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Reexamination Certificate
2003-12-11
2009-11-17
Rao, Manjunath N (Department: 1647)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
C435S007200, C435S007210, C435S007230, C435S325000, C435S366000, C435S371000, C436S501000
Reexamination Certificate
active
07618786
ABSTRACT:
The present invention provides a new method for identifying modulators of human orexin-2 receptor without utilizing recombinantly produced nucleic acid molecules encoding human orexin receptor protein. This method combines and utilizes known methods and cell lines selected for their natural expression of orexin-2 receptors to carry out the methods of the present invention. Exemplary methods of the present invention utilize PFSK-1 cells to produce non-recombinant human orexin-2 receptor protein.
REFERENCES:
patent: 5589351 (1996-12-01), Harootunian
patent: WO 01/85693 (2001-11-01), None
Fults, et al, 1992, J. Neuropathol. Exper. Neurol., 51(3): 272-280.
Huang, et al, 1997, Attenuation of Gi- and Gq-mediated signaling by expression of RGS4 or GAIP in mammalian cells. Proc. Natl. Acad. Sci., 94: 6159-6163.
Chemelli, R.M. et al., “Narcolepsy inorexinKnockout Mice: Molecular Genetics of Sleep Regulation”Cell,1999, 98, 437-451.
Chen, Chiung-Tong et al., “Pressor Effects of Orexins Injected Intracisternally and to Rostral Ventrolateral Medulla of Anesthetized Rats”,Am. J. Physiol.,2000, 278, R692-R697.
Cheng and Prusoff, “Relationship between the Inhibition Constant (k1) and the Concentration of Inhibitor which Causes 50 Per Cent Inhibition (i50) of an Enzymatic Reaction”,Biochemical Pharmacology,1973, 22, 3099-3108.
Conklin, B.R.et al., “Substitution of Three Amino Acids Switches Receptor Specificity of Gqα to that of Giα”,Nature,1993, 363, 274-276.
Fults, D.et al., “Establishment and Characterization of a Human Primitive Neuroectodermal Tumor Cell Line from the Cerebral Hemisphere”,Journal of Neuropathology and Experimental of Neuropathologists,1992, 51(3), 272-280.
Hara, J. et al., “Genetic Ablation of Orexin Neurons in Mice Results in Narcolepsy, Hypophagia, and Obesity”,Neuron,2001, 30, 345-354.
Kirchgessner, A.L. et al., “Orexin Synthesis and Response in the Gut”Neuron,1999, 24, 941-951.
Lin, L. et al., “The Sleep Disorder Canine Narcolepsy is Caused by a Mutation in the Hypocretin (Orexin) Receptor 2 Gene”,Cell,1999, 98, 365-376.
Mignot, E. et al., “Complex HLA-DR and-DQ Interactions Confer Risk of Narcolepsy-Cataplexy in Three Ethnic Groups”,Am. J. Hum. Genet.,2001, 68, 686-699.
Milligan, G. et al., “Chimaeric Gα Proteins” Their Potential Use in Drug Discovery,Trends Pharmacol,1999, 20, 118-124.
Mignot, E. et al., “Narcolepsy and the HLA System”,New England Journal of Medicine,2001, 344, 692.
Nakamura, T. et al., “Orexin-Induced Hyperlocomotion and Stereotypy are Mediated by the Dopaminergic System”,Brain Research,2000, 873 181-187.
Peyron, C. et al., “Neurons Containing Hypocretin (Orexin) project to Multiple Neuronal Systems”,The Journal of Neuroscience,1998, 18(23), 9996-10015.
Peyron, C. et al., “A Mutation in a Case of Early Onset Narcolepsy and a Generalized Absence of Hypocretin Peptides in Human Narcoleptic Brains”Nature Medicine,2000, 6, 991-997.
Piper, D.C. et al., “The Novel Brain Neuropeptide, Orexin-A, Modulates the Sleep-Wake Cycle of Rats”,European Journal of Neuroscience,2000, 12, 726-730.
Samson, W.K. et al., “Cardiovascular Regulatory Actions of the Hypocretins in Brain”Brain Research,1999, 831, 248-253.
Shirasaka, T. et al., “Sympathetic and Cardiovascular Actions or Orexins in Conscious Rats”American J. Physiological Society,1999, 277, R1780-R1785.
Takahashi, N. et al., “Stimulation of Gastric Acid Secretion by Centrally Administered Orexin-A in Conscious Rats”,Biochemical and Biophysical Research Communications,1999, 254, 623-627.
van den Pol, A.N. “Hypothalamic Hypocretin (Orexin): Robust Innervation of the Spinal Cord”,The Journal of Neuroscience,1999, 19, 3171-3182.
Yamanaka, A. et al., “Orexins Activate Histaminergic Neurons via the Orexin 2 Receptor”,Biochemical and Biophysical Research Communications,2002, 290, 1237-1245.
Kane, J.K. et al., “Sensitivity of Orexin-A Binding to Phospholipase C Inhibitors, Neuropeptide Y and Secretin,”Biochemical and Biophysical Research Communications,2000, 272, 959-965.
Lee, J-H et al., “Solution structure of a new hypothalamic neuropeptide, human hypocretin-2/orexin-B,”Eur. J. Biochem.,1999, 266, 831-839.
Gartlon, J.E. et al., “Role of OX1and OX2receptors in the motor activity response to the orexins,”Society for Neuroscience Abstracts,2001, 27(1), 848.
Jaszberenyi, M. et al., “Effects of orexins on the hypothalamic-pituitary-adrenal system,”Journal of Neuroendocronology,2000, 12(12), 1174-1178.
Mignot, E. et al., “Sleeping with the hypothalamus: emerging therapeutic targets for sleep disorders,”Nature Neuroscience, Nature America, Inc.,2002, 5 Suppl., 1071-1075.
Sakurai, T. et al., “Oxerins and oxerin receptors: A family of hypothalamic neuropeptides and G Protein-coupled receptors that regulate feeding behavriour,”Cell,1998, 92, 573-585.
Lovenberg Timothy W.
Sutton Steven W.
Janssen Pharmaceutica N.V.
Rao Manjunath N
Wegert Sandra
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