Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Reexamination Certificate
2007-11-13
2007-11-13
Wessendorf, T. D. (Department: 1639)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
C530S327000, C530S300000, C435S004000, C435S091500, C435S091500, C435S091500
Reexamination Certificate
active
10411336
ABSTRACT:
This invention relates to methods for identifying peptides and other compounds which block or enhance G protein coupled receptor mediated signaling with high affinity and specificity and/or which stabilize a particular conformer of a G protein coupled receptor. Assays, methods of treatment and other methods developed in conjunction with these methods also are disclosed.
REFERENCES:
patent: 4160452 (1979-07-01), Theeuwes
patent: 4256108 (1981-03-01), Theeuwes
patent: 4265874 (1981-05-01), Bonsen et al.
patent: 5034506 (1991-07-01), Summerton et al.
patent: 5733731 (1998-03-01), Schatz et al.
patent: 5880972 (1999-03-01), Horlbeck
patent: 5892014 (1999-04-01), Coughlin et al.
patent: 5955575 (1999-09-01), Peri et al.
patent: 6087186 (2000-07-01), Cargill et al.
patent: 6156511 (2000-12-01), Schatz et al.
patent: 6184223 (2001-02-01), Kahn et al.
patent: 6300312 (2001-10-01), Chemtob et al.
patent: 6617114 (2003-09-01), Fowlkes et al.
patent: 6864060 (2005-03-01), Fowlkes et al.
patent: 6864229 (2005-03-01), Kuliopulos et al.
patent: 2004/0220198 (2004-11-01), Haldar et al.
patent: WO98/19162 (1998-05-01), None
Gates, et al., Affinity Selective Isolation of Ligands from Peptide Libraries Through Display on a lac Repressor “Headpiece Dimer”,J. Mol. Biol., 255, 373-386, 1996.
Lewin, B., “G Proteins May Activate or Inhibit Target Proteins,” Genes VII, Oxford University Press 2000, Chapter 26, pp. 809-811.
Acharya et al., “Transducin-α C-terminal Peptide Binding site Consists of C-D and E-F Loops of Rhodopsin”, J. Biol. Chem., 272(10):6519-6524, 1997.
Adang et al., “The contribution of combinatorial chemistry to lead generation: an interim analysis,” Curr. Med. Chem., 8(9):985-998, 2001 (Abstract Only).
Aris et al., “Structural Requirements for the Stabilization of Metarhodopsin II by the C Terminus of the α subunit of Transducin,” J. Biol. Chem., 276(4):2333-2339, 2001.
Azpiazu et al., “A G Protein γ Subunit-specific Peptide Inhibits Muscarinic Receptor Signaling,” J. Biol. Chem., 274(50):35305-35308, 1999.
Bae et al., “Molecular Determinants of Selectivity in 5-Hydroxytryptamine1BReceptor-G Protein Interactions”, J. Biol. Chem., 272(51):32071-32077, 1997.
Bala et al., “Novel peptidomimics as angiotensin-converting enzyme inhibitors: a combinatorial approach,” Bioorg. Med. Chem., 10(11):3685-91, 2002 (Abstract Only).
Barker et al., “Constitutively Active 5-Hydroxytryptamine2cReceptors Reveal Novel Inverse Agonist Activitiy of Receptor Ligands”, J. Biol. Chem., 269(16):11687-11690, 1994.
Bertin et al., “Functional Expression of the Human Serotonin 5-HT1A Receptor inEscherichia coli”, J. Biol. Chem., 267(12):8200-8206, 1992.
Blahos et al., “A Novel Site on the Gα-protein That Recognizes Heptahelical Receptors”, J. Biol. Chem. 276(5):3262-3269, 2001.
Buck et al., “Role of Dynamic Interactions in Effective Signal Transfer for Gβ Stimulation of Phospholipase C-β2”, J. Biol. Chem., 277(51):49707-49715, 2002.
Cheadle et al., “Identification of a Src SH3 Domain Binding Motif by Screening a Random Phage Display Library”, J. Biol. Chem., 269(39):24034-24039, 1994.
Chidiac et al., “Inverse Agonist Activity of β-Adrenergic Antagonist”, Mol. Pharmacol., 45:490-499, 1994.
Conklin et al., “Carboxyl-Terminal Mutations of Gqαand GsαThat Alter the Fidelity of Receptor Activation”, Mol. Pharmacol., 50:885-890, 1996.
Conklin et al., “Substitution of three amino acids switches receptor specificity of Gqα to that of Giα”, Nature, 363:274-276, 1993.
Copeland, Robert A., “Mechanistic considerations in high-throughput screening”, Analytical Biochem., 320:1-12, 2003.
Costa and Herz, “Antagonists with negative intrinsic activity at δ opioid receptors coupled to GTP-binding proteins”, Proc. Natl. Acad. Sci. USA, 86:7321-7325, 1989.
Costa et al., “Drug Efficacy at Guanine Nucleotide-Binding Regulatory Protein-Linked Receptors: Thermodynamic Interpretation of Negative Antagonism and of Receptor Activity in the Absence of Ligand”, Mol. Pharmacol., 41:549-560, 1992.
Cull et al., “Screening for receptor ligands using large libraries of peptides linked to the C terminus of thelacrepressor”, Proc. Natl. Acad. Sci. USA, 89:1865-1869, 1992..
Cwirla et al., “Peptide Agonist of the Thrombopoietin Receptor as Potent as the Natural Cytokine”, Science, 276:1696-1699, 1997.
Dani, Maria, “Peptide Display Libraries: Design and Construction”, J. of Receptor & Signal Transduction Research, 21(4):469-488, 2001.
Farfel et al., “The Expanding Spectrum of G Protein Diseases”, New Engl. J. Med., 340(13): 1012-1020, 1999.
Flanagan et al., “Advances in understanding gonadotrophin-releasing hormone receptor structure and ligand interactions”, Rev. of Reprod., 2:113-120, 1997.
Ford et al., “Molecular Basis for Interactions of G Protein βy Subunits with Effectors”, Science, 280:1271-1274, 1998.
Francken et al., “Human 5-hydroxytryptamine5AReceptors Activate Coexpressed Giand GoProteins inSpodoptera frugiperda9 Cells”, Mol. Pharmacol., 57:1034-1044, 2000.
Garcia et al., “Transducin-α C-terminal mutations prevent activation by rhodopsin: a new assay using recombinant proteins expressed in cultured cells”, EMBO J., 14(18):4460-4469, 1995.
Gilchrist et al., “Use of Peptides-on-Plasmids Combinatorial Library to Identify High-Affinity Peptides That Bind Rhodopsin”, Methods in Enzymology, 315:388-404, 2000.
Gilchrist et al., “Gα COOH-Terminal Minigene Vectors Dissect Heterotrimeric G Protein Signaling”, Protocols: Science's STKE, 118, 2002, (Abstract only).
Gilchrist et al., “A Dominant-Negative Strategy for Studying Roles of G Proteins in Vivo”, J. of Biol. Chem., 274(10):6610-6616, 1999.
Gilchrist et al., “Antagonists of the Receptor-G Protein Interface Block Gi-coupled Signal Transduction”, J. Biol. Chem., 273(24):14912-14919, 1998.
Glass et al., Agonist Selective Regulation of G Proteins by Cannabinoid CB1and CB2Receptors, Mol. Pharmacol., 56:1362-1369, 1999.
Greco et al. “Cancer Genere Therapy: ‘Delivery, Delivery, Delivery’”, Frontiers in Bioscience, 7:1516-1524, 2002.
Gromoll et al., “Functional and clinical consequences of mutations in the FSH receptor”, Mol. and Cell. Endocrinol., 125:177-182, 1996.
Hall, David A., “Modeling the Functional Effects of Allosteric Modulators at Pharmacological Receptors: An Extension of the Two-State Model of Receptor Activation”, Mol. Pharmacol., 58(6):1412-1423, 2000.
Hamm and Gilchrist, “Heterotrimeric G proteins”, Current Opinion in Cell Biol., 8:189-196, 1996.
Hamm et al., “Site of G protein binding to rhodopsin mapped with synthetic peptides from the alpha subunit”, Science, 241(4867):832-835, 1988. (Abstract only).
Hamm, Heidi E., “The Many Faces of G Protein Signaling,” J. of Biol. Chem. 273(2):669-672, 1998.
Hasegawa et al. “Two Isoforms of the Prostaglandin E Receptor EP3 Subtype Different in Agonist-independent Constitutive Activity”, J. Biol. Chem., 271(4):1857-1860, 1996.
Inanobe et al., “Molecular cloning and characterization of a novel splicing variant of the Kir3.2 subunit predominantly expressed in mouse testis”, J. Physiol., 521.1:19-30,1999.
Kay et al., “Screening Phage-Displayed Combinatorial Peptide Libraries”, Methods, 24:240-246, 2001.
Koivunen et al., “Identification of Receptor Ligands with Phage Display Peptide Libraries”, J. Nucl. Med., 40(5):883-888, 1999.
König, et al., “Three cytoplasmic loops of rhodopsin interact with transducin”, Proc. Natl. Acad. Sci. USA, 86:6878-6882, 1989.
Kosteni
Gilchrist Annette
Hamm Heidi M.
Caden Biosciences
Rothwell, Figg, Ernst & Manbeck pc
Wessendorf T. D.
LandOfFree
Method for identifying modulators of G protein coupled... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for identifying modulators of G protein coupled..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for identifying modulators of G protein coupled... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3804897