Method for identifying inhibitors of soraphen A resistant acetyl

Details Method for identifying inhibitors of soraphen A resistant acetyl Method for identifying inhibitors of soraphen A resistant acetyl

1995-06-06

2000-11-28

Gitomer, Ralph

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

435 29, 435 32, 435 692, C12Q 100, C12Q 118

Patent

active

061533742

ABSTRACT:
An assay to identify inhibitors of soraphen A resistant acetyl-coenzyme A carboxylase comprising measuring the reactivity of acetyl-coenzyme A carboxylase in the presence and in the absence of a compound suspected to inhibit soraphen A resistant acetyl-coenzyme A carboxylase reactivity, and comparing the reactivity measurements to identify inhibitors of soraphen A resistant acetyl-coenzyme A carboxylase.

REFERENCES:
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Roessler, P.G., "Genetic engineering approaches for enchanced production of biodiesel fuel from microalagae", Abstr. Pap. Am. Chem. Soc., 205 Meet., Pt. 2:BTEC29 (1993).
Sikorski, R.S., et al., "A System of Shuttle Vectors and Yeast Host Designed for Efficient Manipulation of DNA in Saccharomyces cerevisiae", Genetics, 122:19-27 (1989).
Vahlensieck, H-F., Ph.D. Thesis, "A Genetic Approach to Determine the Biochemical Targert of the Antimicrobial Agent, Soraphen A", Philosophisch-Naturwissenschaftlichen Fakultat der Universitat Basel (1993).
Vahlensieck H., Idenfication of the Yeast ACC1 Gene Product as the Target of the Polyketide Fungicide Soraphen A, Current Genetics 25, 95-100, 1994.
Beforf, N., Isolation and Structure Elucidation of Soraphen A, A Novel Antifungal Macrolide from Soragnium cellulosum, Liebigs Ann Chem 1017-1021, 1993.

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