Method for identifying individuals at risk for colorectal neopla

Details Method for identifying individuals at risk for colorectal neopla Method for identifying individuals at risk for colorectal neopla

1997-08-19

1999-08-24

Lankford, Jr., Leon B.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 4, 435 71, 435 721, 435 723, 435 29, 435968, C12Q 168, C12Q 100, G01N 33574

Patent

active

059423960

ABSTRACT:
Methods are described for determining in individuals with or without a prior history of colorectal neoplasia whether the individual has had, has, or has a risk of developing colorectal neoplasia. The method involves an assessment of the extent of apoptosis, or programmed cell death, in a colorectal biopsy specimen. Any of numerous methods to measure the extent of apoptosis in the sample is contemplated. For example, normal-appearing mucosa from the distal colon and/or rectum may be obtained from individuals undergoing screening procedures for colorectal cancer. The tissue is subjected to a method that assesses apoptosis, for example, by the extent of broken DNA ends in the nuclei of the cells in the specimen. The extent of apoptosis is expressed as a numerical ratio and indicates the possibility that an individual patient has a premalignant or malignant lesion in his or her colon or the risk for the development of a colorectal neoplasm in the future. Furthermore, the method has utility in determining whether a patient with, or at risk of developing, colorectal neoplasia is responding to prevention or intervention therapy.

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