Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1997-11-07
2000-04-04
Brusca, John S.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 699, 435 914, 4352551, 4353201, 536 234, 536 2374, 536 241, C12Q 168, C12P 2100, C12N 100, C07H 2104
Patent
active
060460008
ABSTRACT:
The invention involves a method of identifying nucleic acid sequences encoding signal peptide-containing proteins. The method features chimeric constructs containing a KRE9 gene that lacks a signal sequence. Yeast containing chimeric KRE9 plasmid constructs that encode signal sequences are selected based on their ability to grow on media in which sucrose is the sole carbon source.
REFERENCES:
patent: 5395922 (1995-03-01), Bjorn et al.
patent: 5525486 (1996-06-01), Honjo et al.
patent: 5536637 (1996-07-01), Jacobs
patent: 5665590 (1997-09-01), Yang
patent: 5712116 (1998-01-01), Jacobs
patent: 5753462 (1998-05-01), Lok
patent: 5789653 (1998-08-01), Skarnes
Orkin et al. Report and recommendations of the panel to assess the NIH investment in research on gene therapy, Dec. 7, 1995.
Wilson, J. Vectors --shuttle vehicles for gene therapy. Clin. Exp. Immunol. vol. 107 (Suppl. 1):31-32, Jan. 16, 1997.
Brown et al., "SKN7, a Yeast Multicopy Suppressor of a Mutation Affecting Cell Wall B-Glucan Assembly . . ." J. of Bacteriology 175(21):6908-6915, 1993.
Brown et al., "The Yeast KRE9 Gene Encodes an O Glycoprotein Involved in Cell Surface B-Glucan Assembly" Mol. and Cell. Biol. 13(10):6346-6356, 1993.
Dijkgraaf et al., "The KNH1 Gene of Saccharomyces cerevisiae is a Functional Homolog of KRE9" Yeast 12:683-692, 1996.
Klein et al., "Selection for genes encoding secreted proteins and receptors" Proc. Nat'l. Acad. Sci. USA 93:7108-7113, 1996.
Nielsen et al., "Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites" Protein Engineering 10(1):1-6, 1997.
Tashiro et al., "Signal Sequence Trap: A Cloning Strategy for Secreted Proteins and Type I Membrane proteins" Science 261:600-603, 1993.
Bossone Steven
Bulawa Christine Ellen
Kuranda Michael Joseph
McCarthy Sean A.
Brusca John S.
Millennium BioTherapeutics, Inc.
Sandals William
LandOfFree
Method for identifying genes encoding signal sequences does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for identifying genes encoding signal sequences, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for identifying genes encoding signal sequences will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-363890