Method for identifying ester coolers

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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C530S350000, C536S023500

Reexamination Certificate

active

07662576

ABSTRACT:
The Transient Receptor Potential Cation Channel, Subfamily A, Member 1 (TRPA1) protein has been identified as an ester cooler receptor and therefore is useful in screening assays for identifying ester coolers, in particular ester coolers with a relative cooling strength which exceeds (−)−menthol.

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patent: WO 2005089206 (2005-09-01), None
NCBI Accession No. Y10601 [gi:4165268] with Revision History—Jun. 30, 1998-Apr. 18, 2005.
NCBI Accession No. NM—007332 [gi:116534989] with Revision History—Dec. 18, 1999-Sep. 25, 2007.
Bianchi et al., “Application of Large-Scale Transiently Transfected Cells to Functional Assays of Ion Channels:Different Targets and Assay Formats”, ASSAY and Drug Development Technologies 2007 5:417-424.
Hinman et al., “TRP channel activation by reversible covalent modification”, Proc Natl Acad Sci 2006 103 (51):19564-19568.
Jordt et al., “Mustard oils and cannabinoids excite sensory nerve fibres through the TRP channel ANKTM1”, Nature 2004: 417:260-265.
Macpherson et al., “Noxious compounds activate TRPA1 ion channels through covalent modification of cysteines”, Nature 2007 445:541-545.
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