Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Reexamination Certificate
1999-06-15
2001-08-07
Jones, W. Gary (Department: 1655)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
C435S006120, C435S254100, C536S023100
Reexamination Certificate
active
06271003
ABSTRACT:
BACKGROUND OF THE INVENTION
(1) Field of the Invention
The invention relates to a method for distinguishing
Cordyceps sinensis
, and more particularly to a laser level apparatus that is easy to use and has multipurpose uses.
(2) Description of the Prior Art
Cordyceps sinensis
(
C. sinensis
) is a complex having a sclerotium and a stroma and is grown from a bug-parasitized fungus that parasitizes in a worm of hepialus armoricanus oberthur. According to the Alexopoulos classification of 1996
, C. sinensis
belongs to the Cordyceps Fr of the Clavicipiyaceae of the Clavicipitales of Ascomycetes. Currently, there are about 400 species found on the planet, which belong to the Cordyceps Fr genus. Among those species, few possess medical ingredients; such as
C. sinensis, C. sobolifera, C. militaris, C. hawkesii
, and so on. Therein,
C. sinensis
is the most popular one. After pharmacological study,
C. sinensis
was found to have active ingredients for curing tumors, kidney inflammation and aging, and for improving immunizability. Therefore,
C. sinensis
has become one of the important topics with studies of medical fumgi.
Due to the important medical potential of
C. sinensis
, healthy foods containing
C. sinensis
ingredients are popular products for health-care and age-care. However, while
C. sinensis
is widely accepted, questions arise to what
C. sinensis
really is. What are actual ingredients of a
C. sinensis
? What characteristics does a genuine
C. sinensis
have? How to identify a real
C. sinensis
? So far, no assured answer for any of the preceding questions is provided. Until now, people studying
C. sinensis
or the related Cordyceps can only work on the species collection, description, and identification. Regarding the medical potential of
C. sinensis
, “existing some metabolic products with some active ingredients for disease-prevention” is the only conclusion that can be provided. Of course, in some literatures, some efforts have been made to further understand the aspect of the Cordyceps, particularly
C. sinensis
, in view of the nature variety of the sexless Cordyceps. However, due to the collection difficulty, identification and reservation of
C. sinensis
, and also due to the difficulty in artificially cultivating the stroma, a clear picture in classification and a genuine relationship between the sex generation and the sexless generation has not been found so far. Hence, the confusion in standardizedly distinguishing
C. sinensis
is still there.
SUMMARY OF THE INVENTION
Since current classification methodologies by configuration, by physiology, or by biochemistry cannot provide a satisfactory and accurate classification of
C. sinensis
; a real
C. sinensis
can't be certainly identified. Therefore, a new method in classifying the Cordyceps is urgently needed. Accordingly, the present invention teaches a method for distinguishing
Cordyceps sinensis
. In view of gene ordering, the difference between
C. sinensis
and other Cordyceps can be determined. Further, the method of the present invention can be applied to distinguish so-called
C. sinensis
or fungi in the market or in the fungi centers. By providing the method of the present invention, specific nature characteristics for
C. sinensis
can be defined. Thereby, a standard and wide-acceptable distinguishing method for
C. sinensis
can be provided.
According to the present invention, the rRNA gene is as an operational target for the distinguishing method, in which the rRNA is the most popular gene in species classification. The rRNA has advantages in a high copy number and a high-degree conservability of evolution. The method of the present invention is first to locate the 18SrRNA of the DNA extracted from a specimen or a fungus, and then to analysis the 18SrRNA by the PCR-RFLP technique widely used in identifying fungi. By using restriction enzyme Cfo I and digesting NS3 and NS6 as the primers, it is found in the digesting polymorphism of the amplification of 18S rRNA gene that
C. sinensis
opposes a total different DNA segment to any other sinensis. This finding can be applied to distinguish
C. sinensis
from other sinensises. Also, thereby, a distinguishing method for C. sinensis can be proposed. The method for distinguishing
C. sinensis
in accordance with the present invention can then become a standard and accurate guideline of identifying
C. sinensis
to the professionals and the related bureau.
REFERENCES:
patent: 5434048 (1995-07-01), Simon et al.
Fulton, C.E. & Brown, A.E., Use of SSU rDNA group-I intron to distinguishMonilinia fructicolafromM.I laxaandM. fructigena, FEMS Micro. Letters 157:307-312, 1997.*
Meyers, R.A., Molecular Biology and Biotechnology, “PCR Technology” pp. 641-648, 1995.
Chen Chih-Shang
Hseu Ruey-Shyang
Birch Stewart Kolasch & Birch, LLP.
Forman B J
Hseu Ruey-Shyang
Jones W. Gary
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