Method for identifying compounds affecting RNA/RNA binding prote

Details Method for identifying compounds affecting RNA/RNA binding prote Method for identifying compounds affecting RNA/RNA binding prote

1997-07-31

1999-12-21

Zitomer, Stephanie

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

530350, 536 221, 937 77, 937 78, 436501, 514 2, C12Q 168, C07K 1400, C07H 2102, G01N 33566

Patent

active

060047496

ABSTRACT:
It has been discovered that a single set of conditions can be used to detect nearly every interaction of RNA binding proteins and RNA molecules. Prior to this discovery it was thought that each specific interaction required separate optimized conditions in order to be detected. Assays employing the disclosed universal conditions are useful for identifying RNA binding proteins that interact with specific RNA molecules of interest, detecting RNA molecules that interact with specific RNA binding proteins of interest, identifying RNA binding proteins active in certain cell types and under certain physiological conditions, identifying specific regions of an RNA molecule that interact with RNA binding proteins. Screening assays employing the disclosed universal conditions are useful for identifying compounds that modulate RNA/RBP interactions of interest.

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