Method for identifying cells committed to apoptosis by determini

Details Method for identifying cells committed to apoptosis by determini Method for identifying cells committed to apoptosis by determini

1995-03-28

1997-04-29

Chin, Christopher L.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 71, 435 72, 435 723, 435 405, 435 4051, 435960, 435968, 436172, 436175, 436536, 436800, 436813, 436824, G01N 3353, G01N 33536, G01N 33574

Patent

active

056248083

ABSTRACT:
A novel approach to study changes in protein tyrosine phosphorylation during apoptosis, and thereby identify cells committed to apoptosis is presented, methods used to study apoptosis and tyrosine phosphorylation at the single cell level are combined to study directly whether apoptosis in hematopoietic cells is associated with changes in cellular phosphotyrosine levels. The changes in cellular phosphotyrosine content strongly correlated with the appearance of features of cell death such as cell shrinkage, DNA fragmentation and loss of membrane integrity.

REFERENCES:
Farr, A.G. et al. Cell, vol. 43, No. 2, pt. 1, pp. 543-550 Dec. 1985.
Otani, H. et al., J. Biol. Chem., vol. 268, No. 30, pp., 22733-22736 Oct. 25, 1993.
Far, D.F. et al., Cytometry, vol. 15, No. 4, pp. 327-334 Apr. 1, 1994.
Yousefi, S. et al., Proc. Natl. Acad. Sci. USA, vol. 91, No. 23, pp. 10868-10872 Nov. 8, 1994.

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