Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2003-10-27
2010-10-19
Carlson, Karen Cochrane (Department: 1652)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C536S024310, C536S023100
Reexamination Certificate
active
07816078
ABSTRACT:
The present invention provides a method for identifying species and subspecies in a biological sample through the selective amplification of segments of nucleic acid that code a target region of the cytoplasmatic beta-actin protein, which is present in all the organisms concerned. The method comprises DNA extraction from the sample; amplification of divergent segments of the cytoplasmatic beta-actin gene by PCR or an equivalent technique, using primers of regions with high evolutionary conservation between species and subspecies; and identification of the amplified segment by comparison of its size in base pairs with a pre-established standard of sizes and/or identification of the amplified segment by DNA sequencing and comparison of the resulting sequence with the specific sequence of each species or subspecies present on a computer database.
REFERENCES:
patent: 5645994 (1997-07-01), Huang
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Rodriguez, M.A. et al., Qualitative PCR for the detection of chicken and pork adulteration in goose and mule duck foie grass, Journal of the Science of Food and Agriculture 83: 1176-1181 (2003).
NG, S.-Y. et al., Evolution of the Functional Human β-Actin Gene and Its Multi-Pseudogene Family: Conservation of Noncoding Regions and Chromosomal Dispersion of Pseudogenes, Molecular and Cellular Biology vol. 5, No. 10, 2720-2732 (1985).
Carlson Karen Cochrane
Gottlieb Rackman & Reisman P.C.
Kosson Rosanne
Laboratorios Dr. F. Echevarne, Analisis, S.A.
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