Method for identifying and using biosynthetic genes for enhanced

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

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4352523, 4353201, C12N 1500, C12N 1510, C12N 1563

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active

051089181

ABSTRACT:
Novel methods and compositions are provided for the enhanced production of secondary metabolites. Methods are provided for identifying sequences which when transformed into secondary metabolite producing hosts, enhance the production of secondary metabolite. The process is exemplified for penicillin. In addition, the P. chrysogenum acyltransferase gene is isolated and sequenced.

REFERENCES:
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Trends in Biotechnology (5) 306-308, 1987, Martin, Cloning of Genes Involved in Penicillin and Cephalosporin Biosynthesis.
Martin and Liras, "Biosynthesis of .beta.-Lactam Antibiotics: Design and Construction of Overproducing Strains" in Trends Biotechnol. (1985) 3(2):39-44.
Carr et al., "Cloning and Expression of the Isopencillin N Synthetase Gene from Penicillium chrysogenum" in Gene (1986) 48:257-266.
Samson, et al., "Isolation, Sequence Determination and Expression in Escherichia coli of the Isopenicillin N Synthetase Gene from Cephalosporium acremonium" in Nature (1985) 318(14):191-194.
Skatrud, et al., "Strain Improvement Studies in Penicillium chrysogenum Using the Cloned P. chrysogenum Isopenicillin N Synthetase Gene and the amdS Gene of Aspergillus nidulans" in SIM News (1987) 37:77.
Maniatis, T., et al., "Molecular Cloning, a laboratory manual" in Cold Spring Harbor Laboratory (1985), pp. 227-228.
Sargent, T. D., et al., "Differential Gene Expression in Gastrula of Xenopus laevis" in Science (1983) 22:135-139.
Hedrick, et al., "Isolation of cDNA Clones Encoding T Cell-Specific Membrane-associated Proteins" in Nature (1984) 308:149-153.
Ramon, et al., "Cloning and Characterization of the Isopenicillin N Synthetase Gene Mediating the Formation of the beta-Lactam Ring in Aspergillus nidulans" in Gene (1987) 57:171-181.

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