Method for identifying and/or isolating genes which code for mem

Details Method for identifying and/or isolating genes which code for mem Method for identifying and/or isolating genes which code for mem

1991-09-06

1994-07-12

Hill, Jr., Robert J.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435 691, 436501, 536 235, C12N 1510

Patent

active

053288386

ABSTRACT:
A method for the analysis and isolation of genes which express membrane receptors was developed. Cells of E. coli transformed with proper vectors, from phage, as well as from plasmids, containing the cDNA coding for human .beta.-adrenergic receptors, are grown on slabs of LB/agar; the bacterial colonies, once transferred onto nitrocellulose filters, show specific activity for (.sup.125 I)-iodocyanopindolol (ICYP). The .beta.-adrenergic receptors expressed in bacteria retain their pharmacologic characteristics after being transferred on filters.

REFERENCES:
P.N.A.S. 80:1194-1198, Mar. 1983, Young et al. Efficient isolation of genes by using antibody probes.
P.N.A.S. 85:7551-7555, Oct. 1988, Marullo et al. Human B.sub.2 Adrenagic Receptors Expressed in Escherchia coli Membranes Retain Their Pharmacological Properties.

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