Method for identifying and isolating cells expressing leukocyte

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 72, 435325, 435374, G01N 3353, C12N 506

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active

057767076

ABSTRACT:
Cells expressing Leukocyte Adhesion Molecule-1 (LAM-1) are identified by reaction with an anti-LAM1-3 monoclonal antibody produced by a hybridoma cell line made by the fusion of NS-1 myeloma cells with spleen cells obtained from mice immunized with a LAM-1 cDNA transfected cells. The identification methods are expected to be clinically useful in the diagnosis of disease such as AIDS, which are associated with cells expressing LAM-1 surface protein.

REFERENCES:
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T.F. Tedder et al., Isolation and Chromosomal Localization of cDNAs Encoding . . . , J. Exp. Med., 170: 123-133 (1989).
T.F. Tedder et al.; "Human antigen-specific memeory T cells express homing Receptor (LAM-1) . . . ," Eur. J. Immunol., 20: 1351-1355 (1990).
T.F. Tedder et al., "Expression of The Human Leukocyte Adhesion Molecule, LAM-1," J. Immunol., 144: 532-540 (1990).
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Picker, et al., "The Neutrophil Selectin LECAM-1 Presents Carbohydrate Ligands to the Vascular Selectins ELAM-1 and GMP-140," Cell 66: 921-933 (1991).
Kishimoto, et al., "Antibodies Against Human Neutrophil LECAM-1 (LAM-1/Leu-8/DREG-56 Antigen) and Endothelial Cell ELAM-1 Inhibit a Common CD18-Independent Adhesion Pathway in Vitro," Blood 78: 805-811 (1991).
Jutila, et al., "Characterization of a Functionally Important and Evolutionally Well-Conserved Epitope Mapped to the Short Consensus Repeats of E-Selectin and L-Selectin," J.Exp. Med. 175: 1565-1573 (1992).
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Tsang et al. J Clin Immunol 20:159-165 (1986) Abstract only.
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