Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1994-09-30
1997-02-25
Nucker, Christine M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 5, 435 72, 435 78, 435 792, 435 793, 435 794, 435 795, 435 15, 435 35, 435974, 436516, G01N 33569
Patent
active
056058020
ABSTRACT:
In accordance with the present invention, there are provided novel assays which allow the identification of compounds which block the ability of lentiviruses to infect non-dividing cells. Compounds discovered employing the invention methods can be employed to block the ability of HIV to infect non-dividing cells. In accordance with another aspect of the present invention, novel antibodies have been developed which are specifically immunoreactive with the phosphorylated form of HIV-1 MA. In accordance with yet another aspect of the present invention, novel kinases which phosphorylate HIV-1 MA have been discovered.
REFERENCES:
Gallay et al. "HIV-1 Infection of Nondividing Cells: C-Terminal Tyrosine Phosphorlylation of the Viral Matrix Protein Is a Key Regulator" Cell, vol. 80, No. 3 (Feb. 10, 1995), pp. 379-388. QH573.
Burnette et al. "Phosphorylation of HIV-1 gag Proteins by Protein Kinase C" Th e Journal of Biological Chemistry, vol. 268, No. 12 (Apr. 25, 1993), pp. 8698-8703, QP501.J7.
Ruksen et al., "Nonradioactive Assays of Protein-Tyrosine Kinase Activity Using Anti-phosphotyrosine Antibodies" Methods in Enzymology 200:98-107 (1991).
Ferrari and Thomas, "Micro-and Macropurification Methods for Protein Kinases" Methods in Enzymology 200:159-169 (1991).
Schwedler et al., "The nuclear localization signal of the matrix protein of human immunodeficiency virus type 1 allows the establishment of infection in macrophages and quiescent T lymphocytes" Proc. Natl. Acad. Sci. 91:6992-6996 (1994).
Ronnstrand and Heldin, "Purification of Platelet-Derived Growth Factor .beta. Receptor from Porcine Uterus" Methods in Enzymology 200:371-388 (1991).
Yu et al., "Vpx of simian immunodeficiency virus is localized primarily outside the virus core in mature virions" Journal of Virology 67:4386-4390 (1993).
Zack et al., "Incompletely reverse-transcribed human immunodeficiency virus type 1 genomes in quiescent cells can function as intermediates in the retroviral life cycles" Journal of Virology 66:1717-1725 (1992).
Zack et al., "HIV-1 entry quiesecnt primary lymphocytes: Molecular analysis reveals a labile, latent viral structure" Cell 61:213-222 (1990).
Zhou et al., "Identification of a membrane-binding domain within the amino-terminal region of human immunodeficiency virus type 1 Gag protein which interacts with acidic phospholids" Journal of Virology 68:2556-2569 (1994).
Trono, Didier, "Partial Reverse Transcripts in Virions from Human Immunodeficiency and Murine Leukemia Viruses" Journal of Virology 66:4893-4900 (1992).
Schwedler et al., "vif Is Crucial for Human Immunodeficiency Virus Type 1 Proviral DNA Synthesis in Infected Cells" Journal of Virology 67:4945-4955 (1993).
Sahal et al., "Solid-Phase Protein-Tyrosine Kinase Assay" Methods in Enzymology 200:90-97 (1991.
Stochaj and Silver, "A conserved phosphoprotein that specifically binds nuclear localization sequences is involved in nuclear import" Journal of Cell Biology 117:473-482 (1992).
Thelen et al., "Regulation by phosphorylation of reversible association of a myristoylated protein kinase C substrate with the plasma membrane" Nature 351:320-322 (1991).
Trono et al., "HIV-1 Gag mutants can dominantly interfere with the replication of the wild-type virus" Cell 59:113-120 (1989).
Varshavsky, A. "Naming a targeting signal" Cell 64:13-15 (1991).
Walker et al., "Translocation of pp60.sup.c-src from the plasma membrane to the cytosol after simulation by the platelet-derived growth factor" The Journal of Biological Chemistry 268:19552-19558 (1993).
Yu et al., "The matrix protein of human immunodeficiency virus type 1 is required for incorporation of viral envelope protein into mature virions" Journal of Virology 66:4966-4971 (1992).
Yu et al., "The C terminus of human immunodeficiency virus type 1 matrix protein is involved in early steps of the virus life cycle" Journal of Virology 66:5667-5670 (1992).
Yu et al., "Open Reading Frame vpr of Simian Immunodeficiency Virus Encodes a Virion-Associated Protein" Journal of Virology 64:5688-5693 (1990).
Yuan et al., "Mutations in the N-terminal region of human immunodeficiency virus type 1 matrix protein block intracellular transport of the Gag precursor" Journal of Virology 67:6387-6394 (1993).
Paxton et al., "Incorporation of Vpr into human immunodeficiency virus type 1 virions: requirement for the p6 region of gag and mutational analysis" Journal of Virology 67:7229-7237 (1993).
Richardson et al., "Nuclear location signals in polyoma virus large T" Cell 44:77-85 (1986).
Rihs and Peters., "Nuclear transport kinetics depend on phosphorylation-site-containing sequences flanking the karyophilic signal of the Simian virus 40 T-antigen" EMBO 7:1479-1484 (1989).
Roe et al., "Integration of murine leukemia virus DNA depends on mitosis" EMBO Journal 12:2099-2108 (1993).
Royer et al., "Functional domains of HIV-1 gag-polyprotein expressed in baculovirus-infected cells" Virology 184:417-422 (1991).
Schultz et al., "Fatty acylation of proteins" Annual Review of Cell Biology 4:611-647 (1988).
Schwedler et al., "vif is crucial for human immunodeficiency virus type 1 proviral DNA synthesis in infected cells" Journal of Virology 67:4945-4955 (1993).
Sharova and Bukrinskaya., "p17 and p17-containing gag precursors of input human immunodeficiency virus are transported into the nuclei of infected cells" Aids Research and Human Retroviruses 7:303-306 (1991).
Spina et al., "The importance of nef in the induction of human immunodeficiency virus type 1 replication from primary quiescent CD4 lymphocytes" Journal of Experimental Medicine 179:115-123 (1994).
Michaud and Goldfarb, "Most nuclear proteins are imported by a single pathway" Experimental Cell Research 208:128-136 (1993).
Miller et al., "The human immunodeficiency virus-1 nef gene product: A positive factor for viral ionfection and replication in primary lymphocytes and macrophages" Journal of Experimental Medicine 179:101-113 (1994).
Moll et al., "The role of phosphorylation and the CDC28 protein kinase in cell cycle-regulated nuclear import of the S. cerevisiae transcription factor SW15" Cell 66:743-758 (1991).
Morin et al., "Nuclear localization of the adenovirus DNA-binding protein: requirement for two signals and complementation during viral infection" Mollecular and Cellular Biology 9:4372-4380 (1989).
Ogawa et al., "Mutational analysis of the human immunodeficiency virus vpr open reading frame" Journal of Virology 63:4110-4114 (1989).
Okuno et al., "70-kDa heat-shock cognate protein colocalizes with karyophilic proteins into the nucleus during their transport in vitro". Experimental Cell Research 206:134-142 (1993).
Pandey and Parnaik, "Identification and characterization of nuclear location signal-binding protein in the nuclear envelopes" Biochimica et Biophysica ACTA 1063:81-89 (1991).
Lang., et al "Importance of vpr for infection of Rhesus monkeys with simian immunodeficiency virus" Journal of Virology 67:909-912 (1993).
Lavallee et al., "Requirement of the Pr55gag precursor for incorporation of the Vpr product into human immunodeficiency virus type 1 with viral particles" Journal of Virology 68:1926-1934 (1994).
Lavallee et al., "HIV-1 HxBc2 strain encodes a truncated vpr gene product of 78 amino acids" Journal of Acquired Immune Deficiency Syndromes 6:529-530 (1993).
Levy et al., "Induction of cell differentiation by human immunodeficiency virus 1 vpr" Cell 72:541-550 (1993).
Lewis and Emerman, "Passage through mitosis is required for oncoretroviruses but not for the human immunodeficiency virus" Journal of Virology 68:510-516 (1994).
Lewis et al., "Human immunodeficiency virus infection of cells arrested in the cell cycle" EMBO Journal 11:3053-3058 (1992).
Li et al., "Intracellular distribution of a nuclear localization signal binding protein" Experimental Cell Research 202:355-365 (1992).
Lu et al., "Human immunodeficiency virus type 1 viral protein R localization in infected cells and virions" Journal of Virology 67:6542-6550 (1993).
Meltzer et al., "Role of mononuclear phagocytes in the pathogenesis of human immunodeficiency virus infection" An
Aiken Christopher R.
Gallay Philippe A.
Swingler Simon M.
Trono Didier P.
Nucker Christine M.
Ramos Robert T.
Reiter Stephen E.
Stucker Jeffrey
The Salk Institute for Biological Studies
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