Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving transferase
Reexamination Certificate
2002-02-27
2008-07-15
Hutson, Richard G (Department: 1652)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving transferase
C435S194000, C435S006120, C435S005000, C435S091300, C435S069200
Reexamination Certificate
active
07399605
ABSTRACT:
The present invention relates to the molecular biology and virology of the hepatitis C virus (HCV). An object of the present invention is a method to reproduce in vitro the RNA-dependent RNA polymerase activity of HCV that makes use of sequences contained in the HCV NS5B protein.
REFERENCES:
patent: 5981247 (1999-11-01), Hagedorn et al.
patent: 6383768 (2002-05-01), De Francesco et al.
patent: 0 464 287 (1990-12-01), None
patent: 0 463 848 (1991-06-01), None
patent: WO 94/05809 (1994-03-01), None
patent: WO 95/22985 (1995-08-01), None
patent: WO 97/12033 (1997-04-01), None
Al et al., Expression and characterization of the NS5B (RNA-dependent RNA polymerase) gene of hepatitis C virus, Hepatology, vol. 22, No. 4 Pt. 2, pp. 331A, 1995.
Tomei et al., NS3 is a serine protease required for processing of hepatitis C virus polyprotein, J. of Virology, vol. 67, No. 7, pp. 4017-4026, 1993.
Bartenschlager et al., Kinetic and structural analyses of hepatitis C virus polyprotein processing, J. of Virology, vol. 68, No. 8, pp. 5045-5055, 1994.
Lin et al., Hepatitis C virus NS3 serine proteinase trans-cleavage requirements and processing kinetics, J. of Virology, vol. 68, No. 12, pp. 8147-8157, 1994.
Miller et al., Hepatitis C virus shares amino acid sequence similarity with pestiviruses and flaviviruses as well as members of two plant virus supergroups, Proc. Natl. Acad. Sci. USA, vol. 87, pp. 2057-2061, 1990.
Behrens et al., Identification and properties of the RNA-dependent RNA polymerase of hepatitis C virus, The EMBO Journal, vol. 15, No. 1, pp. 12-22, 1996.
Bartholomeusz et al., Use of a flavivirus RNA-dependent RNA polymerase assay to investigate the antiviral activity of selected compounds, Antiviral Research, vol. 24, pp. 341-350, 1994.
Grun et al., Dissociation of NS5 from cell fractions containing west nile virus-specific polymerase activity, Journal of Virology, vol. 61, No. 11, pp. 3641-3644, 1987.
Chu et al., Characterization of kunjin virus RNA-dependent RNA polymerase: reinitiation of synthesis in vitro, Virology, vol. 157, pp. 330-337, 1987.
Grun et al., Characterization of west nile virus RNA-dependent RNA polymerase and cellular terminal adenylyl and uridylyl transferase in cell-free extracts, Journal of Virology, vol. 60, No. 3, pp. 1113-1124, 1986.
Bartholomeusz et al., Synthesis of dengue virus RNA in vitro: initiation and the involvement of proteins NS3 and NS5, Arch Virol, vol. 128, pp. 111-121, 1993.
Lohmann et al., Biochemical properties of hepatitis C virus NS5B RNA-dependent RNA polymerase and identification of amino acid sequence motifs essential for enzymatic activity, Journal of Virology, vol. 71, No. 11, pp. 8416-8428, 1997.
Chung, R. et al. “Identification and Characterization of a Hepatitis C Virus-Specific RNA-Dependent RNA Polymerase Activity From Extracts of Infected Liver Tissue”, Hepatology, 1992, vol. 16, No. 4 Part 2, Abstract 350.
Grakoui, A. et al. “Expression and Identification of Hepatitis C Virus Polyprotein Cleavage Products”, Journal of Virology, 1993, vol. 67, pp. 1385-1395.
Handschuh, G. et al. “Bacterial expression and purification of hepatitis C virus capsid proteins of different size”, Journal of Hepatology, 1995, vol. 22, pp. 143-150.
Kato, N. et al. “Molecular cloning of the human hepatitis C virus genome from Japanese patients with non-A, non-B hepatitis”, 1990, vol. 87, pp. 9524-9528.
Matsurra, Y. et al. “The molecular biology of hepatitis C”, Seminars in Virology, 1993, vol. 4, pp. 297-304.
Neufeld, K. et al. “Identification of Terminal Adenylyl Transferase Activity of the Poliovirus Polymerase 3Dpol”, Journal of Virology, 1994, vol. 68, pp. 5811-5818.
Plotch, S. et al. “Purification and Properties of Poliovirus RNA Polymerase Expressed inEscherichia coli”, Journal of Virology, 1989, vol. 63, pp. 216-225.
Shirai, M. et al. “Induction of Cytotoxic T Cells to a Cross-Reactive Epitope in the Hepatitis C Virus Nonstructural RNA Polymerase-Like Protein”, Journal of Virology, 1992, vol. 66, pp. 4098-4106.
Takamizawa, A. et al. “Structure and Organization of the Hepatitis C Virus Genome Isolated from Human Carriers”, Journal of Virology, 1991, vol. 65, pp. 1105-1113.
Tsutsumi, M. et al. “Detection of Antigens Related to Hepatitis C Virus RNA Encoding the NS5 Region in the Livers of Patients with Chronic Type C Hepatitis”, Hepatology, 1994, vol. 19, pp. 265-272.
Chung, R. et al. “Parenterally Transmitted Hepatitis: Viruses, Vaccines, and Antiviral Therapy”, Comprehensive Therapy, 1993, vol. 19, pp. 163-173.
Lide, D. “CRC Handbook of Chemistry and Physics”, 81st Edition 200-2001, pp. 7-1.
Behrens Sven-Erik
De Francesco Raffaele
Tomei Licia
Heber Sheldon O.
Hutson Richard G
Instituto Di Ricerche Di Biologia Molecolare P. Angeletti S.p.A.
Wenk Melissa B.
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