Method for identification of medically relevant fungi

Details Method for identification of medically relevant fungi Method for identification of medically relevant fungi

2006-02-16

2010-02-09

Calamita, Heather G (Department: 1637)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S091100, C435S091200, C536S024330

Reexamination Certificate

active

07659067

ABSTRACT:
Multiple species of fungi in an environment can be identified in one sample by extracting and purifying fungal DNA in the sample. PCR is then performed followed by cloning the amplifed DNA and transforming the DNA into bacteria for purposes of growing the organisms. Colonies of the growth containing transformed bacteria are then chosen on the basis of coloration. Plasmids from the chosen colonies were then purified and the DNA is analyzed to identify fungi present in the sample.

REFERENCES:
patent: 6235890 (2001-05-01), Morrison et al.
patent: 6872523 (2005-03-01), Iwen et al.
Wu et al. J. Environ. Monit. vol. 4:377-382. 2002.
Borneman et al., Applied and Environmental Microbiology vol. 66:4356-4360. 2000.

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