Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1994-01-24
1996-12-24
Fleisher, Mindy
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435 4, 435 34, 436 63, 436 64, 436161, 436173, 436174, 436177, 436178, 436813, 530350, 530395, 530416, 530418, C12Q 102, G01N 3348, C07K 118, C07K 130
Patent
active
055872972
ABSTRACT:
The present invention provides methods for identifying molecular components (e.g. proteins and/or lipids) that are characteristic of vascular endothelia associated with a particular disease and are not also present in (i) normal (non-disease associated) vascular endothelia or (ii) non-disease associated vascular endothelia subjected to altered conditions that accompany the disease but are not unique to the disease. The methods include isolating plasma membrane fractions from disease-associated vascular endothelial cells, from normal (nondisease-associated) vascular endothelial cells and from nondisease-associated vascular endothelial cells subjected to at least one of the altered conditions; purifying and resolving molecular components from these membrane fractions; comparing the components resolved from each of the foregoing vascular endothelial cell sources and identifying components that are present in disease-associated endothelial cells and absent from the normal or otherwise conditioned endothelial cells. The resulting endothelial cell membrane components that are unique to the disease state can be used for diagnostic and/or therapeutic purposes.
REFERENCES:
patent: 3007878 (1961-11-01), Alexander et al.
patent: 4925922 (1990-05-01), Byers et al.
patent: 5059523 (1991-10-01), Rettig et al.
patent: 5169774 (1992-12-01), Frankel et al.
patent: 5227307 (1993-07-01), Bar-Or et al.
patent: 5281700 (1994-01-01), Schnitzer et al.
Burrow et al., Canc. Res. 52: 5954-62, 1992.
Chaney and Jacobson, J. Biol. Chem. 258: 10062, 1983.
Clark and West, Electrophoresis 12: 590, 1991.
Gerritsen et al., Adv. Cell Culture 6: 35-67, 1988.
Gougas et al. J. Biol. Chem. 265: 8361-4, 1990.
Kumar et al., in Angiogenesis in Health & Disease 227: 63-78, 1992, Plenum Press NATO, Asi Series.
Mizrachi et al., Cell & Tissue Res. 256: 365-72, 1989.
Murray et al., Radiother. Oncol. 16: 221-34, 1989.
Ogawa et al., Proc. Natl. Acad. Sci. USA. 88: 9897-9901, 1991.
Patton, Biochim & Biophys Acta 816: 83-92, 1985.
Patton et al., J. Cell Physiol. 134: 37-46, 1988.
Rettig et al., Proc. Natl. Acad. Sci., USA. 89: 10832-6, 1992.
Sambuy et al., Proc. Natl. Acad. Sci. USA. 85: 1529-33, 1988.
Schnitzer, Am. J. Physiol. 262: H246-H254, 1992.
Schrappe et al., Canc. Res. 51: 4986-93, 1991.
Stolz et al., In vitro Cell Dev. Biol. 27A: 169-182, 1991.
Wang et al., Int'l J. Can. 54: 363-70, 1993.
Weidner et al., NEJM 324: 1-7, 1992.
Noguchi et al. "Identification & Partial Purification of A Novel Tumor Derived Protein That Induces Tissue Factor On Cultured Human EndoThelial Cells" Biochem Biophys. Res Comm 160 (1) 222-227 1989.
Jacobson Bruce S.
Schnitzer Jan E.
Degen Nancy J.
Fleisher Mindy
The Regents of the University of California
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