Method for hybridizing nucleic acids using single-stranded nucle

Details Method for hybridizing nucleic acids using single-stranded nucle Method for hybridizing nucleic acids using single-stranded nucle

1989-10-24

1995-09-12

Fleisher, Mindy B.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

536 243, 935 77, 935 78, C12Q 168, C07H 2104, C12N 1500

Patent

active

054496039

ABSTRACT:
The present invention relates to an improved method for hybridizing polynucleotides with complementary nucleic acid sequences. Specifically, it relates to a method of increasing the specificity of a polynucleotide hybridization reaction in the presence of single-stranded nucleic acid binding protein.

REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
Muriyappa et al., Proc. Natl. Acad. Sci. USA, v. 81; May 1984, 2757-61.
Christiansen et al., J. Mol. Biol., 115: 441-54 (1977).

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