Method for HLA Typing

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 912, 536 243, 935 77, 935 78, C12Q 168, C07H 2104

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active

055455263

ABSTRACT:
The invention provides an improved method for typing specific HLA sequences and other genetic sequences exhibiting similar polymorphism may be detected by sequence specific amplification (SSA). In essence, the primers used to carry out the amplification are chosen to represent sequences that distinguish one allele from another. As a result, the allele is detected if amplification occurs, and is absent if no amplification occurs. This technique can eliminate the need to conduct sequence-specific probe hybridization in order to distinguish among closely similar alleles, and can, in particular, allow resolution of complementary pairs of naturally-occurring HLA alleles which have the same polymorphisms but located on different individual alleles of each pair.

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