Method for HLA typing

Details Method for HLA typing Method for HLA typing

1993-04-08

1997-12-30

Vogel, Nancy T.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, 536 2433, C12Q 168, C12P 1934, C07H 2104

Patent

active

057028850

ABSTRACT:
A method for HLA typing by amplification of a sample followed by sequence-specific oligonucleotide hybridization with a labelled oligonucleotide probe provides for both positive and negative controls. Control sequences representing known allelic polymorphisms at the locus in question are subjected to the labelled probe along with the sample. This method reduces errors and improves the chance of obtaining a successful tissue match, as is vital in the case of tissue transplants, particularly bone marrow transplants. Probes and PCR primers useful in HLA-DR typing are also provided.

REFERENCES:
patent: 4582788 (1986-04-01), Erlich
patent: 4683202 (1987-07-01), Mullis
patent: 4835098 (1989-05-01), Orr et al.
Baxter-Low et al., J. Clin. Invest., vol. 84, Aug. 1989, pp. 613-618.
Saiki et al, Nature, vol. 324, 13; Nov., 1986, pp. 163-166.
Bugawan et al., J. of Immuno., vol. 141, No. 12, Dec., 1988, pp. 4024-4030.
Scharf et al., Science, vol. 233, Sep. 1986, pp. 1076-1078.
Angelini et al, Proc. Natl. Acad. Sci. USA, vol. 83, pp. 4489-4493, Jun. 1986.
Ronningen et al., Human Immunology 24, pp. 165-173, 1989.
Ucla et al, J. Clin. Invest., vol. 80, pp. 1155-1159, 1987.
Le Gall et al., Proc. Natl. Acad. Sci. U.S.A., vol. 83, pp. 7836-7840, Oct., 1986.
Human Immunology 24, First Domain Sequence . . . Gorski, 145-149 (1989).
Nature, Polymorphism of Human Ia Antigens . . ., vol. 322, No. 6074, pp. 67-70, Gorski et al. (1986).
Proc. Natl. Acad. Sci. USA, Generation of Single-Stranded . . . , vol. 85, pp. 7652-7656 (1988).
Human Immunology 24, DNA Typing of DRw6 Subtypes . . . Tiercy et al., pp. 1-14 (1989).
Proc. Natl. Acad. Sci. USA, Identification and Distribution of . . . , vol. 85, pp. 198-202 (1988).
Am. J. Hum. Genet., HLA-DR Typing . . . , Cox et al., vol. 43, No. 6, pp. 954-963 (1988).
Sniki et al., Proc. Natl. Acad. Sci USA 86:6230-6234 (1989).
Fernandez-Vina et al. Human Immunol., 28:51-64 (1990).
Saiki et al., New Engl. J. of Medicine, vol. 319(9): 537-541 (1988).

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