Method for HLA-DR typing of total human lymphocyte sample

Details Method for HLA-DR typing of total human lymphocyte sample Method for HLA-DR typing of total human lymphocyte sample

1982-04-02

1984-09-11

Marantz, Sidney

Chemistry: analytical and immunological testing

Involving iga, igd, ige, or igm

424 11, 436519, 436548, 436821, G01N 3350, G01N 3354, G01N 3380

Patent

active

044710569

ABSTRACT:
An HLA-DR typing test based on lymphocytotoxicity in which a vital dye-labeled total human lymphocyte sample, such as a sample of peripheral blood lymphocytes, is incubated with HLA-DR antisera, a monoclonal antibody against T cells, and complement and the DR type is determined based upon the resultant cytotoxicity as measured by the fluorescence of B cells surviving the incubation.

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patent: 4328183 (1982-05-01), Rosenfield
patent: 4403042 (1983-09-01), Henry
patent: 4420558 (1983-12-01), De Mey
J. Van Wauwe et al., Immunology, 42 (1), 157-164 (Jan. 1981).
van Rood, J. J. et al., Nature, 262, 795-797 (1976).
Report of the Eighth International Histocompatibility Workshop; Articles by A. van Leeuwen et al., pp. 278-279; by C. Bland et al., pp. 280-282; by A. Longo et al., pp. 283-284; by A. H. Johnson et al., pp. 285-286; and by J. A. Danilovs et al., pp. 287-288.

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