Method for highly sensitive hybridization of nucleic acids,...

Details Method for highly sensitive hybridization of nucleic acids,... Method for highly sensitive hybridization of nucleic acids,...

2006-05-30

2006-05-30

Whisenant, Ethan (Department: 1634)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C536S023100, C536S024300

Reexamination Certificate

active

07052842

ABSTRACT:
A method for hybridizing nucleic acids, which includes an annealing step of preparing a first single stranded nucleic acid fragment immobilized on a surface of an immobilizing material and a second single stranded nucleic acid fragment labeled with fluorescence or radioisotope and forming a complementary double strand from the first single stranded nucleic acid fragment and the second single stranded nucleic acid fragment, and an enzyme treatment step. In the annealing step, the complementary double strand is formed by performing a temperature gradient processing performed from a high temperature area to a low temperature area, and in the enzyme treatment step, a noncomplementary nucleic acid portion contained in the complementary double stand is recognized and cleaved by adding endonuclease. This method has high measurement sensitivity and the operation is simple.

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Yoshinari Fukuda, “Southern Northern Hybridization Ho”, Medical Technology, vol. 26, No. 3, pp. 239-244, 1998, abstract only.
Yoshinari Fukuda, “Southern Northern Hybridization Ho”, Medical Technology, vol.26 No. 3, pp. 239-244, 1998.

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