Method for highly sensitive detection of single protein...

Details Method for highly sensitive detection of single protein... Method for highly sensitive detection of single protein...

2007-04-04

2009-08-11

Gakh, Yelena G (Department: 1797)

Chemistry: analytical and immunological testing

Optical result

With fluorescence or luminescence

C436S164000

Reexamination Certificate

active

07572640

ABSTRACT:
Disclosed are methods, kits, and compositions for the highly sensitive detection of molecules. The methods, kits, and compositions are useful in determining concentrations of molecules in samples to levels of 1 femtomolar, 1 attomolar, or lower. The methods, kits, and compositions also allow the determination of concentration over a wide range, e.g., 7-log range, without need for sample dilution.

REFERENCES:
patent: 4071298 (1978-01-01), Falconer
patent: 4172227 (1979-10-01), Tyrer et al.
patent: 4243318 (1981-01-01), Stohr
patent: 4251733 (1981-02-01), Hirleman, Jr.
patent: 4768879 (1988-09-01), McLachlan et al.
patent: 4793705 (1988-12-01), Shera
patent: 4927265 (1990-05-01), Brownlee
patent: 4979824 (1990-12-01), Mathies et al.
patent: 5002389 (1991-03-01), Benser
patent: 5041733 (1991-08-01), Noguchi et al.
patent: 5094594 (1992-03-01), Brennan
patent: 5108179 (1992-04-01), Myers
patent: 5138170 (1992-08-01), Noguchi et al.
patent: 5209834 (1993-05-01), Shera
patent: 5269937 (1993-12-01), Dollinger et al.
patent: 5480614 (1996-01-01), Kamahori
patent: 5540494 (1996-07-01), Purvis, Jr. et al.
patent: 5543838 (1996-08-01), Hosier et al.
patent: 5547849 (1996-08-01), Baer et al.
patent: 5571410 (1996-11-01), Swedberg et al.
patent: 5603351 (1997-02-01), Cherukuri et al.
patent: 5605662 (1997-02-01), Heller et al.
patent: 5633503 (1997-05-01), Kosaka
patent: 5645702 (1997-07-01), Witt et al.
patent: 5653859 (1997-08-01), Parton et al.
patent: 5653939 (1997-08-01), Hollis et al.
patent: 5658413 (1997-08-01), Kaltenbach et al.
patent: 5681751 (1997-10-01), Begg et al.
patent: 5682038 (1997-10-01), Hoffman
patent: 5716825 (1998-02-01), Hancock et al.
patent: 5730187 (1998-03-01), Howitz et al.
patent: 5746901 (1998-05-01), Balch et al.
patent: 5755942 (1998-05-01), Zanzucchi et al.
patent: 5770029 (1998-06-01), Nelson et al.
patent: 5793485 (1998-08-01), Gourley
patent: 5798222 (1998-08-01), Goix
patent: 5807677 (1998-09-01), Eigen et al.
patent: 5858195 (1999-01-01), Ramsey
patent: 5863801 (1999-01-01), Southgate et al.
patent: 5949532 (1999-09-01), Schrof et al.
patent: 5955028 (1999-09-01), Chow
patent: 5989402 (1999-11-01), Chow et al.
patent: 5999250 (1999-12-01), Hairston et al.
patent: 6033628 (2000-03-01), Kaltenbach et al.
patent: 6041515 (2000-03-01), Ally et al.
patent: 6049380 (2000-04-01), Goodwin et al.
patent: 6071478 (2000-06-01), Chow
patent: 6114180 (2000-09-01), Doth et al.
patent: 6131101 (2000-10-01), Maitino et al.
patent: 6140048 (2000-10-01), Muller et al.
patent: 6177277 (2001-01-01), Soini
patent: 6208815 (2001-03-01), Seidel et al.
patent: 6211955 (2001-04-01), Basiji et al.
patent: 6249341 (2001-06-01), Basiji et al.
patent: 6280960 (2001-08-01), Carr
patent: 6309886 (2001-10-01), Ambrose et al.
patent: 6355420 (2002-03-01), Chan
patent: 6386219 (2002-05-01), Barth et al.
patent: 6388746 (2002-05-01), Eriksson et al.
patent: 6403338 (2002-06-01), Knapp et al.
patent: 6403947 (2002-06-01), Hoyt et al.
patent: 6473176 (2002-10-01), Basiji et al.
patent: 6495104 (2002-12-01), Unno et al.
patent: 6506609 (2003-01-01), Wada et al.
patent: 6532067 (2003-03-01), Chang et al.
patent: 6533553 (2003-03-01), Caren
patent: 6537437 (2003-03-01), Galambos et al.
patent: 6582903 (2003-06-01), Rigler et al.
patent: 6599436 (2003-07-01), Matzke et al.
patent: 6608680 (2003-08-01), Basiji et al.
patent: 6689323 (2004-02-01), Fisher et al.
patent: 6710871 (2004-03-01), Goix
patent: 6766817 (2004-07-01), Da Silva
patent: 6783992 (2004-08-01), Robotti et al.
patent: 6802342 (2004-10-01), Fernandes et al.
patent: 6811668 (2004-11-01), Berndt et al.
patent: 6816257 (2004-11-01), Goix
patent: 6918404 (2005-07-01), Dias da Silva
patent: 6977305 (2005-12-01), Leung et al.
patent: 7066586 (2006-06-01), Da Silva
patent: 2002/0030812 (2002-03-01), Ortyn et al.
patent: 2002/0167665 (2002-11-01), Yeung et al.
patent: 2003/0029995 (2003-02-01), Mullins et al.
patent: 2003/0078737 (2003-04-01), Keys et al.
patent: 2003/0124592 (2003-07-01), Puskas
patent: 2003/0222007 (2003-12-01), Gu et al.
patent: 2004/0166514 (2004-08-01), Puskas
patent: 2004/0214211 (2004-10-01), Gilmanshin et al.
patent: 2005/0221408 (2005-10-01), Nalefski et al.
patent: 2006/0004188 (2006-01-01), Leung et al.
patent: 2006/0078915 (2006-04-01), Fuchs et al.
patent: 2006/0078998 (2006-04-01), Puskas
patent: 2006/0099638 (2006-05-01), Leung et al.
patent: 2006/0160209 (2006-07-01), Larson et al.
patent: 2006/0228747 (2006-10-01), Fuchs et al.
patent: WO 90/10876 (1990-09-01), None
patent: WO 99/55461 (1999-11-01), None
patent: WO 2005/089524 (2005-09-01), None
patent: WO 2005/089524 (2006-04-01), None
Nalefski, et al. Single-molecule counting of macromolecular complexes in real time: a novel approach to quantify transcription factor- DNA and antibody-antigen interactiosn. Faseb Journal. 2004; 18(8): C176.
Rigler. Fluorescence correlations, single molecule detection and large number screening. Applications in biotechnolgy. J. Biotechnol. 1995; 41(2-3):177-86.
Panchuk-Voloshina, et al. Alexa dyes, a series of new fluorescent dyes that yield exceptionally bright, photostable conjugates. J Histochem Cytochem. Sep. 1999;47(9):1179-88.
Alex Fluor Dyes Handbook. Simply the Best and Brightest: Fluorescent Dyes and Conjugates. Invitrongen. Copyright 2005. Molecular Probes. 1-33.
Alexa Flour Dyes. Succinimidyl Esters. Revised Jan. 4, 2006; 1-5.
Ambrose, et al. Single molecule fluorescence spectroscopy at ambient temperature. Chemical Reviews. 1999; 99(10): 2929-56.
Anazawa, et al. Electrophoretic quantitation of nucleic acids without amplification by single molecule imaging. Anal. Chem. 2002; 2002; 74(19): 5033-38.
Becker, et al. Three-dimensional photogrammetric particle-tracking velocimetry. Preparing for the Future. 1995; 5(3). Available at http://esapub.esrin.esa.it/pff/pffv5n3/beckv5n3.htm (7 pages).
Bieschke, et al., Ultrasensitive detection of pathological prion protein aggregates by dual-color scanning for intensely fluorescent targets. Proc. Natl Acad Sci USA. 2000; 97(10): 5468-5473.
Bouchon, et al. Cutting edge: inflammatory responses can be triggered by TREM-1, a novel receptor expressed on neutrophils and monocytes.The Journal of Immunology.2000; 164(10): 4991-1995.
Brinkmeier, et al. Two-beam cross-correlation: a method to characterize transport phenomena in micrometer-sized structures.Anal. Chem.1999; 71(3): 609-616.
Castro, et al. Fluorescence detection and size measurement of single DNA molecules.Anal. Chem.1993; 65(7): 549-852.
Castro, et al. Single molecule detection: applications to ultrasensitive biochemical analysis.Applied Optics.1995; 34(18): 3218-3222.
Castro, et al. Single-molecule detection of specific nucleic acid sequences in unamplified genomic DNA.Anal. Chem.1997; 69(19):3915-3920.
Castro, et al. Single-molecule electrophoresis.Anal. Chem.1995; 67(18):3181-3186.
Castro, et al. Ultrasensitive, direct detection of a specific DNA sequence ofBacilus antracisin solution.The Analyst.2000; 125: 9-11.
Chan, et al. DNA mapping using microfluidic stretching and single-molecule detection of fluorescent site-specific tags. Genome Res. 2004; 14(6):1137-46.
Chen, et al. Single-molecule detection in capillary electrophoresis: molecular shot noise as a fundamental limit to chemical analysis.Anal. Chem.1996; 68(4): 690-696.
Cohen, et al. Rapid separation and purification of oligonucleotides by high-performance capillary gel electrophoresis.Proc Natl Acad Sci USA.1988; 85(24): 9660-9663.
D'Antoni, et al. Rapid quantitative analysis using a single molecule counting approach. Anal Biochem. 2006; 352(1):97-109.
Dovichi, et al. Laser-induced fluorescence of flowing samples as an approach to single-molecule detection in liquids.Anal. Chem.1984; 56(3): 348-354.
Dunbar, et al. Quantitative, multiplexed detection of bacterial pathogens: DNA and protein applictions of Luminex LabMap system. J Microbiol Methods. 2003; 53(2): 245-252.
Effenhauser, et al. Integrated capillary electrophoresis on flexible silicone microdevices: analysis of DNA restriction fragments and detection of single DNA molecules on microchips.Anal. Ch

Affiliated with

Also associated with

Rating

Method for highly sensitive detection of single protein... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for highly sensitive detection of single protein..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for highly sensitive detection of single protein... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-4058583