Method for highly expressing recombinant glucose...

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor

Reexamination Certificate

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C435S320100, C435S190000, C536S023100

Reexamination Certificate

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07741100

ABSTRACT:
The present invention provides a method for highly expressing a recombinant FAD-GDH protein derived from filamentous fungi, protein obtained by the method, and a regent for measuring glucose using the protein. According to the invention, the FAD-GDH can be highly expressed by altering DNA sequence coding for a signal peptide of FAD-GDH gene isolated fromAspergillus oryzae. FAD-GDH can be stably produced by adjusting pH of 7.1 to 7.3 during culture production.

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