Method for high specific bioproductivity of .alpha.,.omega.-alka

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound

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C12P 744

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active

060664800

ABSTRACT:
This invention provides a low-cost method of producing .alpha.,.omega.-alkanedicarboxylic acids. Particular bioconversion conditions result in highly efficient conversion of fatty acid, fatty acid ester, or alkane substrates to diacids. Candida tropicalis AR40 or similar yeast strains are grown in a medium containing a carbon source and a nitrogen source at a temperature of 31.degree. C. to 38.degree. C., while additional carbon source is continuously added, until maximum cell growth is attained. Within 0-3 hours of this point, substrate is added to the culture to initiate conversion. An .alpha.,.omega.-alkanedicarboxylic acid made according to this method is also provided.

REFERENCES:
patent: 5254466 (1993-10-01), Picataggio et al.
patent: 5620878 (1997-04-01), Picataggio et al.
"Microbial Production of Long-Chain Dicarboxylic Acids from n-Alkanes", Agricultural and Biologiccal Chemistry, vol. 35, No. 13, Jan. 1, 1971 pp. 2033-2042.
"Antifungal Properties of Alpha, Omega-Alkanedicarboxylic acids and Their Dimethyl Esters", Can J. Microbiol., vol. 22, 1976, pp. 1198-1201.

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