Method for high-resolution two-dimensional electrophoresis and d

Details Method for high-resolution two-dimensional electrophoresis and d Method for high-resolution two-dimensional electrophoresis and d

1993-12-20

1995-04-18

Niebling, John

Chemistry: electrical and wave energy

Processes and products

Electrostatic field or electrical discharge

2041828, 2041829, 204299R, C07K 328

Patent

active

054075467

ABSTRACT:
For high-resolution two-dimensional electrophoresis comprising the steps of carrying out, on a gel base, at first a first separation process in a first direction and then a second separation process in a direction vertical to the first direction by a technique different from that of the first separation, for example by molecular screening, it is proposed to use as a basis a dry gel consisting of a single piece and extending in the two dimensions and to hydrate the dry gel selectively in a strip-like area for carrying out the isoelectric separation in the first dimension, while the remaining gel area retains its dry gel configuration, to then perform the separation in the first dimension in the selectively hydrated area, and to hydrate thereafter the remaining electrophoresis area of the gel for carrying out the second separation process.

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