Liquid purification or separation – Processes – Liquid/liquid solvent or colloidal extraction or diffusing...
Patent
1998-10-30
2000-02-15
Therkorn, Ernest G.
Liquid purification or separation
Processes
Liquid/liquid solvent or colloidal extraction or diffusing...
210656, 435 6, 536 254, 935 78, B01D 1508
Patent
active
060248785
ABSTRACT:
Mixtures of dsDNA fragments are separated by Matched Ion Polynucleotide Chromatography (MIPC) using an isocratic mobile phase to elute polynucleic acid from an MIPC column. The use of isocratic elution conditions provides a marked improvement in the separation of dsDNA fragments compared to gradient elution conditions. Isocratic elution can also be used to effect an improved separation of heteroduplex and homoduplex mixtures when the chromatography is performed under partially denaturing conditions. In addition, dsDNA fragments are bound to the stationary phase under isocratic conditions until a solvent concentration is reached which releases fragments of a particular base pair length range. This separation process is different from the equilibrium partitioning process observed under gradient elution conditions.
REFERENCES:
patent: 5024934 (1991-06-01), Lee
patent: 5100547 (1992-03-01), Hardiman et al.
patent: 5459039 (1995-10-01), Modrich et al.
patent: 5585236 (1996-12-01), Bonn et al.
patent: 5654144 (1997-08-01), Mann et al.
patent: 5698400 (1997-12-01), Cotton et al.
patent: 5772889 (1998-06-01), Gjerde
patent: 5795976 (1998-08-01), Oefner et al.
Apffel et al, Applications of HPLC For The Analysis of Double Stranded DNA Use of Wide Pore Silica Based Materials, ISPP (Oct. 1997) pp. 1-5.
Bernardi, Chromatography of Nucleic Acids on Hydroxyapatite columns Methods of Enzymology, vol. XXI, pp. 94-147, 1971.
Berti, Dissertion, Untersuchungen Zur Ionenpaar-Umkehrphasen-Chromatographie Von DNA, Jun. 1996, pp. 52-53.
Coenen et al. Optimization of The Separation of The PR and SP Diasteromers of Phsphate-Methylated DNA and RNA Dinuleotides, Journal of Chromatography, 596, 59-66, (1992).
Cooper et al. An Estimate of Unique DNA Sequence Heterozygosity in The Human Genome, Hum Genet, vol.69, pp. 201-205 (1985).
Cotton et al, Slowly by Surely Towards Better Scanning for Mutations, Tig, vol. 13 No. 2: 43-46 (Feb. 1997).
Doris et al. Quantitive Analysis of Gene Expression by Ion Pair HPLC, Handout of DHPLC Workshop, Stanford University (Mar. 17, 1997) pp. 1-40.
Fritz et al. High-Performance Liquid Chromatography in Polynucleotide Synthesis, Biochemistry, 17, 7, pp. 1257-1267, (1978).
Furst et al. Simultaneous Determination of Myocardial Nucleotides, Nucleosides, Purine Bases and Creative Phosphate by Ion-Pair High-Performance Liquid Chromatography, J. Chromatography, 578, 39-44, Jul. 1, 1992.
Green et al. Preparative Purification F Sypercoiled Plasmid DNA for Therapeutic Applications BioPharm, 10:5 pp. 52-62, May 1997.
Green et al. HPLC Purification of Synthetic Oligodeoxribonucleotides Containing Base-and Backbone-Modified Sequences, BioTechniques 19:5, pp. 836-841 (Nov. 1993).
Guyer et al. How is the Human Genome Project Doing, and What Have we Learned So Far?, Proc. Natl. Acad. Sci., vol. 92, pp. 10841-10848, (1995).
Hayward-Lester et al, Accurate and Absolute Quantitative Measurement of Gene Expression by Single-Tube RT-PCR and HPLC, Genome Research, No. 5: 494-499 (1995).
Heftman, Chromatography, 5th Edition, Journal of Chromatography Library --vol. 51A, Elsevier, pp. A299-A300, 1992.
Hirabayashi et al. Size-Dependent Chromatographic Separation of Double-Stranded DNA Which is Not Based on Gel Permeation Mode, Analytical biochemistry, 178, 336-341, 1989.
Huber et al, High-Resolution Liquid Chromatography of Oligonucleotides on Nonporous Alkylated Styrene-Divinylbenzene Copolymers, Analytical Biochemistry, 212: 351-358 (1993).
Huber et al, Rapid Analysis of Biopolymers on Modified Non-Porous Polystryene-Divinylbenzene Particles, Chromatographia, vol. 37 No. 11/12: 653-658 (Dec. 1993).
Ip et al. Separation O Nucleosides and Nucleotides By Reversed-Phase HPLC with Volatile Buffers Allowing Sample Recovery, Analytical Biochemistry, 147, 180-185, (1985).
Kwiatkowski et al. Use of RP Ion Pair Chromatography to Fraction and Purify DNA Fragments and Monomeric Components of RNA, Acta Chemica scandinavica B. 38, p, 721-733, (1984).
Liu et al, Denaturing High Performance Liquid Chromatography (DHPLC) Used in the Detection of Germline and Somatic Mutaions, Nucleic Acid Research, vol. 26 No. 6: 1396-1400 (1998).
Moriyama et al. New RP HPLC Column for Oligonucleotide Separation, Journal of Chromatography, 445, 225-223, (1988).
Taylor, Graham R., Laboratory Methods for the Detection of Mutations and Polymorphisms in DNA, 18-22, CRC Press, Boca Raton, New York (1997).
Underhill et al, A Pre-Columbian Y Chromosome-Specific Transition and its Implications for Human Evolutionary History, Proc. Natl. Acad. Sci., 93: 196-200 (Jan. 1996).
Underhill et al, Detection of Numerous Y Chromosome Biallelic Polymorphisms By Denaturing High-Performance Liquid Chromatography, Genome Research, No. 7: 996-1005 (1997).
Vtorushna et al. Study of Diasteromers of Non-Ionic Oligonucleotide Analogues. VI. Separation of Disasteromers of Ethyl Phosphotriesters O Octanucleotide d(GCCAAACA) by Means of HP Complementary Chromatography, Bioorg. Khim. 18, 1, 92-99 (1992).
Zon et al. High Performance Liquid Chromatography in Biotechnology, Hancock (ed.) John Wiley & Sons, New York, NY, 1990, pp. 301-398.
Tashlitskii et al. Optimization of Conditions for Ion-Pair HPLC of Oligonucleotides Bioorg. Khim., 23 (9), 732-741 (1997) Biosis Abstract No. 01070821.
Hewlett-Packard, Zorbax Stable Bond Zorbax Eclipse Reverse Phase HPLC Columns, Product Specification undated pp. 1-7.
Transgenomic, Inc. Technical Note General Description: DNASep undated pp. 1-8.
Djordjevic et al, HPLC Separation of Oligonucleotides in Isocratic and Temperature-Programming Mode, Analytical Chemistry, 70: 1921-1925 (1998).
Li et al., Strategies for Faster Gradient Chromatography, LC-GC, vol. 16 No. 5 (May 1998) pp. 468-476.
Snyder et al. Gradient Elution in Reversed-Phase HPLC, Anal. Chem. (1983) 55: 1412A-1430A, No. 14.
Fele Robert M. Hae
Gjerde Douglas T.
Taylor Paul D.
Therkorn Ernest G.
Transgenomic Inc.
Walker William B.
LandOfFree
Method for high resolution liquid chromatographic separation of does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for high resolution liquid chromatographic separation of , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for high resolution liquid chromatographic separation of will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1903090