Method for growing stem cells on genetically modified...

Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Method of co-culturing cells

Reexamination Certificate

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C435S375000, C435S377000, C435S455000

Reexamination Certificate

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07816136

ABSTRACT:
A method for growing stem cells comprising the steps of aproviding stem cells with supporters said supporters being genetically modified in order to provide externally regulatable interactions between the supporters and the stem cells; supporters and stem cells are interchangeable upon genetic modification and interaction;applying an external signal for starting or stopping the interactions.

REFERENCES:
patent: 5922927 (1999-07-01), Bujard et al.
patent: 0 753 574 (1997-01-01), None
patent: WO 97/37009 (1997-10-01), None
Sturtz et al. Tetracycline-Regulatable Expression Vectorss Tightly Regulate In Vitro Gene Expression of Secreted Proteins. Gene. 1998, vol. 221, pp. 279-285.
Tao et al. Evidence for Transdifferentiation of Human Bone Marrow-Derived Stem Cells: Recent Progress and Controversies. Pathology. 2003, vol. 35, pp. 6-13.
Manfred Gossen et al., “Tight Control of Gene Expression in Mammalian Cells By Tetracyline-Responsive Promoters”, Proc. Natl. Acad. Sci. USA, vol. 89, pges. 5547-5551, Jun. 1992.

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