Method for growing eukaryotic cells using soft agar assay and ki

Details Method for growing eukaryotic cells using soft agar assay and ki Method for growing eukaryotic cells using soft agar assay and ki

1994-02-08

1994-12-06

Robinson, Douglas W.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

4352401, 4352403, 43524031, 435810, 435975, C12N 500

Patent

active

053710100

ABSTRACT:
A method for cultivating eukaryotic cells, particularly bone marrow cells, which utilizes a gel system having a gel strength low enough to allow the cells to settle into the gel during cultivation. The gel system is preferably formed from a mixture of methylcellulose and modified agarose. Further, the gel system comprises a soft agar assay and kit for growing the bone marrow cells. The gel is transparent and is present in a single uniform layer with a thickness of 0.5 to 4 mm.

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