Method for growing Cordyceps sinensis on a substrate and...

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Fungi

Reexamination Certificate

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C435S254100, C435S256800, C435S440000, C435S449000, C435S454000

Reexamination Certificate

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07407795

ABSTRACT:
Two different strains ofCordyceps sinensisare placed at two different locations in a medium containing purified rattle snake venom and the two strains are allowed to grow until they meet in a boundary zone where a hybrid strain is formed due to exchange of genetic material. The hybrid strain is allowed to grow and is harvested and can be analyzed for the presence and quantity of desired medicinal substances. The amount of N6-(2-hydroxyethyl)-adenosine in aCordyceps sinensisstrain or product sample is a reliable indicator of the overall health benefiting qualities of the strain or sample.Strains ofCordyceps sinensis, are grown in a substrate at 20 to 22° C. at sea level atmospheric pressure for 28 to 30 days in diffuse light, and thereafter in an atmosphere containing approximately 50% of oxygen of the sea level atmosphere, at approximately 3° C. with exclusion of light, for approximately 15 to 20 weeks to provide aCordyceps sinensisproduct that has the characteristic analytical signature of wildCordyceps sinensisand includes substantial quantities of the health benefiting compounds of the strain found in the wild.

REFERENCES:
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Zhu, et al. The Scientific Rediscovery of an Ancient Chinese Herbal Medicine:Cordyceps sinensisPart I, The Journal of Alternative and Complementary Medicine (1998) p. 289-303. vol. 4 No. 3, Mary Ann Liebert, Inc.
Zhu, et al. The Scientific Rediscovery of a Precious Ancient Chinese Herbal Regimen:Cordyceps sinensisPart II, The Journal of Alternative and Complementary Medicine, (1998), p. 429-457, vol. 4, No. 4, Mary Ann Liebert, Inc.
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Hsu, et al. A Comparison of the Chemical Composition and Bioactive Ingredients of the Chinese Medicinal Mushroom DongChongXiaCao, its Counterfeit and Mimic, and Fermented Mycelium ofCordyceps sinensis, Food Chemistry, (2002), p. 463-469, vol. 78, Elsevier Science, Ltd.
Furuya, et al. N6-(2-Hydroxyethyl) Adenosine, A Biologically Active Compound From Cultured Mycelia ofCordycepsandIsariaSpecies, Phytochemistry, (1983), pp. 2509-2512, vol. 22, No. 11,Perfamon Press Ltd., Great Britain.
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Acta Scientiarum Naturalium Universitatis Sunyatseni, Molecular IdentificationCordyceps sinensis.pdf, (1999), vol. 38, No. 1, p. 01-0121-123, 1995-2005 Tsinghua Tongfang Optical Disc Co., Ltd. (English abstract).
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