Method for growing acid-producing bacteria

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Process involving micro-organisms of different genera in the...

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435139, 435244, 435245, 4352529, 4352534, 4352536, 435885, 426 36, 426 38, 426 39, 426 43, C12P 3900, C12P 138, A23C 912, C12R 146

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051167379

ABSTRACT:
A method is disclosed for growth acid-producing bacterial cultures, such as diary cultures, wherein the culture is selected to contain a urease-producing strain of bacteria and the medium used for the culturing contains added urea. During culturing the urease hydrolyzes the urea to acid-neutralizing ammonia which limits the pH drop of the medium, thereby producing cultures of higher bacterial activity. The urea-containing culture media can also be employed with bacterial cultures which do not produce urease, providing urease is added to the culture medium during growth of the bacteria.

REFERENCES:
patent: 4675193 (1987-06-01), Boudreaux
patent: 4713341 (1987-12-01), Bily
patent: 4797289 (1989-01-01), Reddy
Tinson et al. "Austral. J. Dairy Tech" Mar. 1982, p. 14.
Thunell, "pH-Controlled Starter: A Decade Reviewed", Cultured Dairy Products Journal, Aug. 1988, pp. 10-16.
Suzuki, et al., Appl. Environ. Microb. 37:379-382 (1979).
Juillard, V., et al., Can. J. Microbiol., 34:818-822 (1988), with translation.

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