Method for glycosylating hydroxylysine residues

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

Reexamination Certificate

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C435S041000, C435S183000, C435S189000, C435S252300, C435S320100, C536S023200

Reexamination Certificate

active

10276825

ABSTRACT:
This invention relates to a multifunctional enzyme having lysyl hydroxylase and glycosyltransferase activity and to the use of the enzyme in glycosylating hydroxylysine residues. In particular this invention relates to a method for producing glycosyltransferase activity, which comprises that a nucleotide sequence encoding lysyl hydroxylase and glycosyltransferase activity is introduced and expressed in a chosen host and the protein product is recovered from the host cell or from the culture medium.

REFERENCES:
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patent: WO 00/61755 (2000-10-01), None
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