Method for genome mining for secreted protein genes

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C435S004000, C435S005000, C435S069100, C435S320100, C435S091500, C435S091500, C435S091500, C536S023100, C536S023200

Reexamination Certificate

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07067252

ABSTRACT:
The present invention relates to a method for screening for compounds secreted by an organism, comprising(a) raising antibodies against secreted products of a donor organism,(b) providing a gene library from the donor organism,(c) cloning the gene library into a suitable host organism,(d) expressing the cloned genes in the host organism, and(e) detecting positive clones, which upon expression of the cloned genes secretes a compound, using the antibodies of (a) to identify such positive clones.The invention also relates to compounds, nucleotide sequences and microorganisms identifiable by said method.

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patent: 5665585 (1997-09-01), Torkkeli et al.
patent: 6165718 (2000-12-01), Borchert et al.
patent: WO 89/08114 (1989-09-01), None
Rehman et al (Molecular and Biochemical Parasitology, 97 (1998) 55-68).
Lueking et al., Analytical Biochemistry 270, 103-111 (1999).

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