Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – Via agrobacterium
Reexamination Certificate
2007-03-06
2007-03-06
McElwain, Elizabeth (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
Via agrobacterium
C800S298000, C435S410000, C435S419000, C435S420000, C435S430100
Reexamination Certificate
active
10362019
ABSTRACT:
The invention refers to a method to transform and obtain plants of woody trees, particularlyEucalyptus spp., transformed by the introduction of exogenous genes of interest into their cells. The method makes use of sterilized seeds, which are co-cultivated withAgrobacteriumcells containing a transference plasmid (tDNA), and any gene of interest. The method used for the transformation employs sonication to increase the efficiency of bacteria penetration within teguments and seed embryo tissues.
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Harold N. Trick et al., SAAT: sonication-assistedAgrobacterium-mediated transformation, Transgenic Research, 1997, vol. 6, pp. 329-336.
Harold N. Trick et al., Induction of Somatic Embryogenesis and Genetic Transformation of Ohio Buckeye (Aeculus Glabra Willd.), In Vitro Cellular and Developmental Biology-Plant, 1999, vol. 35, pp. 57-80.
Harold N. Trick et al., SAAT: sonication-assistedAgrobacterium-mediated transformation, Transgenic Research, 1997, vol. 6, pp. 329-336.
Gonzáles Esteban R.
Labate Carlos A.
Labate Monica T. V.
Helmer Georgia
McDermott Will & Emery LLP
McElwain Elizabeth
Suzano Bahia Sul Papel e Celulose S.A.
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