Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1992-07-09
1995-03-14
Elliott, George C.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
4351723, 5303891, C12N 1502, C12N 1506, A61K 3900
Patent
active
053977033
ABSTRACT:
The present invention relates to a method of generating antibodies directed against cell surface antigens. The method uses as immunogen recombinant insect cells into which have been transfected coding regions for a molecule containing a cell surface antigen. Host animals are immunized with these transfected insect cells to generate antibodies directed against the cell surface antigen. Antibody-producing cells from the host animal are used to generate monoclonal antibody-producing hybridoma cells. Sera and hybridoma supernatants can be tested for the presence of antibodies against the surface antigen, using the transfected cells in a screening assay.
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Conroy Leah B.
de Boer Mark
Blackburn Robert P.
Cetus Oncology Corporation
Elliott George C.
Meyers Thomas C.
Savereide Paul B.
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