Radiant energy – Ionic separation or analysis – Methods
Reexamination Certificate
2008-08-05
2010-10-26
Souw, Bernard E (Department: 2881)
Radiant energy
Ionic separation or analysis
Methods
C250S281000, C250S288000, C435S006120, C435S041000, C435S042000, C436S015000, C436S019000, C436S056000
Reexamination Certificate
active
07820963
ABSTRACT:
A method for identifying a biological analyte that is affected by a stressor is disclosed in which two substantially identical biological samples are provided, with a first sample being a control sample and a second sample being an experimental sample. The control sample is grown with a nutrient having an isotope of a first atom, whereas the experimental sample is grown with a nutrient having a second isotope of the first atom. The experimental sample is grown with a stressing agent and regimen. The samples are admixed, and the formed composite is mass spectroscopically assayed for analyte peaks. The ratio of first isotope to second isotope is determined for the peaks, as is a sample median isotopic ratio. The ratio for assayed analyte peaks is compared with the median ratio. An analyte whose isotopic ratio significantly deviates from the median ratio is an analyte affected by the stressing agent.
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Husch Blackwell Welsh & Katz
Metabolic Alayses, Inc.
Souw Bernard E
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