Method for generation and use of isotopic patterns in mass...

Radiant energy – Ionic separation or analysis – Methods

Reexamination Certificate

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C250S281000, C250S288000, C435S006120, C435S041000, C435S042000, C436S015000, C436S019000, C436S056000

Reexamination Certificate

active

07820963

ABSTRACT:
A method for identifying a biological analyte that is affected by a stressor is disclosed in which two substantially identical biological samples are provided, with a first sample being a control sample and a second sample being an experimental sample. The control sample is grown with a nutrient having an isotope of a first atom, whereas the experimental sample is grown with a nutrient having a second isotope of the first atom. The experimental sample is grown with a stressing agent and regimen. The samples are admixed, and the formed composite is mass spectroscopically assayed for analyte peaks. The ratio of first isotope to second isotope is determined for the peaks, as is a sample median isotopic ratio. The ratio for assayed analyte peaks is compared with the median ratio. An analyte whose isotopic ratio significantly deviates from the median ratio is an analyte affected by the stressing agent.

REFERENCES:
patent: 5087815 (1992-02-01), Schultz et al.
patent: 5462946 (1995-10-01), Mitchell et al.
patent: 5912178 (1999-06-01), Porter et al.
patent: 6355416 (2002-03-01), Abramson
patent: 6849396 (2005-02-01), Schneider
patent: 6940065 (2005-09-01), Graber et al.
patent: 6952818 (2005-10-01), Ikeuchi
patent: 7045296 (2006-05-01), Parker et al.
patent: 7368710 (2008-05-01), Garner et al.
patent: 2004/0091943 (2004-05-01), Schneider
patent: 2007/0275483 (2007-11-01), Liotta et al.
patent: 2009/0039247 (2009-02-01), Beecher
patent: 2009/0124518 (2009-05-01), Beecher
Hellerstein,Metabolic Engineering6:85-100 (2004).
Williams et al.,Experimantal Cell Research69:106-112 . (197I).
Wu et al.,Anal Biochem336:164-171 (2005).
Katajamaa et al., BMCBioinformatics2005, 6 : 179 doi:10.1186/1471-2105-6-179.
Rögnvaldsson et al., 2004J. Chrom. B, 807:209-215; doi:10.1016/j.jchromb.2004.04.010.
Johannsen, 1911American Naturalist45:129-159.

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