Method for generating overexpression of alleles in genes of...

Multicellular living organisms and unmodified parts thereof and – Method of making a transgenic nonhuman animal

Reexamination Certificate

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C800S013000, C800S022000

Reexamination Certificate

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10263367

ABSTRACT:
Methods for generating and using novel overexpression activity alleles of a gene in any organism, especiallyDrosophilia, are provided. Such alleles may be utilized in screening assays, and used to generate dominant-negative forms of bacterial toxins.

REFERENCES:
Noll et al., “New functions of theDrosophila rhomboidgene during embryonic and adult development are revealed by a novel genetic method, enhancer piracy,” Development 120: 2329-2338, 1994.
Yu et al., “TheDrosophila decapentaplegicand short gastrulation genes function antagonistically during adult wing vein development,” Development 122: 4033-4044, 1996.
Duffy, “GAL4 System inDrosophila: A Fly Geneticist's Swiss Army Knife,”Genesis, 34:1-15 (2002).
Reiter, et al., “A Systematic Analysis of Human Disease-Associated Gene Sequences inDrosophila melanogaster,”, Gen.Res., 11:1114-1125 (2001).

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