Method for generating new flow cytometry data files...

Data processing: measuring – calibrating – or testing – Measurement system – Performance or efficiency evaluation

Reexamination Certificate

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Reexamination Certificate

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07321843

ABSTRACT:
A method that generates new flow cytometry data files with a potentially infinite number of dimensions is described. First two or more separate flow cytometry data files containing information about events measured in different aliquots from the same sample are fused. Then, the magnitude of the values and a corresponding uncertainty measure of the differing variables between one or more of the original data files which have been fused are estimated, for each individual event contained in the fused data file, for which that variable was not directly measured in the flow cytometer. The new data file containing both data actually measured in the flow cytometer for each event of the individual original data files fused and the estimated data for those parameters not measured in a group of events from one or more of the original data files are reconstructed multidimensionally.

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