Method for generating metal chelating affinity ligands

Details Method for generating metal chelating affinity ligands Method for generating metal chelating affinity ligands

2011-08-02

2011-08-02

Therkorn, Ernest G (Department: 1778)

Liquid purification or separation

Processes

Liquid/liquid solvent or colloidal extraction or diffusing...

C210S656000, C210S198200, C210S502100, C502S401000, C502S439000

Reexamination Certificate

active

07988858

ABSTRACT:
The present invention relates to a method for generating at least one polydentate metal chelating affinity ligand, which method comprises the steps of(a) providing at least one scaffold defined by the general formula (I): H2N—(X1)n—S—S—(X2)m—CH2—NH2wherein X1and X2irrespective of each other are carbon atoms or heteroatoms, and n and m irrespective of each other are integers of 1 to 5;(b) providing at least one polydentate metal chelating affinity ligand arm, optionally in a form wherein the metal chelating functionalities of at least one arm are protected, on each scaffold by derivatisation of the nucleophilic NH2groups of the scaffold;(c) reducing the disulfide bond of the derivatised scaffold; and, if required(d) deprotecting the functionalities of the ligand arm(s) provided in step (b). In the most preferred embodiment, the reduction of the disulfide bond and the deprotection step is performed essentially simultaneously.

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