Method for generating birnavirus from synthetic RNA transcripts

Details Method for generating birnavirus from synthetic RNA transcripts Method for generating birnavirus from synthetic RNA transcripts

1996-09-05

1999-02-16

Woodward, Michael P.

Drug, bio-affecting and body treating compositions

Antigen, epitope, or other immunospecific immunoeffector

Virus or component thereof

4241841, 4241861, 4241851, 4242041, 424816, 424826, 435 711, 4352351, 435236, 435237, 435238, 435239, 4353201, 536 2372, A61K 3912, A61K 3900, A61K 3938

Patent

active

058717447

ABSTRACT:
A system for the generation of live Birnavirus such as infectious bursal disease virus (IBDV), a segmented double-stranded (ds)RNA virus of the Birnavirdae family, using synthetic transcripts derived from cloned DNA has been developed. Independent full-length cDNA clones were constructed which contained the entire coding and non-coding regions of RNA segments A and B of IBDV, respectively. Synthetic RNAs of both segments were produced by in vitro transcription of linearized plasmids with T7 RNA polymerase. Transfection of Vero cells with combined plus-sense transcripts of both segments generated infectious virus as early as 36 hours post-transfection. The development of a reverse genetics system for dsRNA viruses will greatly facilitate studies of the regulation of viral gene expression pathogenesis, and design of a new generation of live and inactivated vaccines.

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