Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1992-08-21
1994-11-22
Kepplinger, Esther M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 23, 435 681, 436501, 436518, 530338, 530343, G01N 33558, C12P 2106, C12Q 137
Patent
active
053668626
ABSTRACT:
The invention allows the generation and screening of a large population of peptides for the presence of peptides which bind a particular macromolecule or macromolecular complex with high affinity, and further allows the favored net synthesis of analyzable quantities of such peptides, by using as the "trap" a macromolecule or macromolecular complex for which binding of the peptide is desired. The starting mixture is preferably spiked with a peptide having some affinity for the target macromolecule so that mutation of the spike or "lead" peptide is favored. The development of improved binding peptides through scrambling may be dynamically monitored by initially binding the target with an insolubilized ligand, and then looking for an increase in the concentration of the target in the soluble phase as a result of the displacement of the reference ligand by scrambled peptides.
REFERENCES:
patent: 3544426 (1970-12-01), Snellman
patent: 3649457 (1972-03-01), Westman
patent: 3843444 (1974-10-01), Likhite
patent: 3855196 (1974-12-01), Matsukawa
patent: 3972773 (1976-08-01), Isowa
patent: 3993636 (1976-11-01), Maubois
patent: 4086136 (1978-04-01), Isowa
patent: 4116768 (1978-09-01), Isowa
patent: 4119493 (1978-10-01), Isowa
patent: 4165311 (1979-08-01), Isowa
patent: 4256836 (1981-03-01), Isowa
patent: 4320196 (1982-03-01), Morihara et al.
patent: 4320197 (1982-03-01), Morihara
patent: 4339534 (1982-07-01), Johansen
patent: 4442204 (1984-04-01), Greenquist et al.
patent: 4521514 (1985-06-01), Oyama
patent: 4806473 (1989-02-01), Johansen et al.
Stuart R. Stone et al., Kinetics of the Inhibition of Thrombin by Hirudin, Biochemistry, 25:4622-4628, 1986.
John M. Maraganore et al., Anticoagulant Activity of Synthetic Hirudin Peptides, The Journal of Biological Chemistry, 264:8692-8698, 1989.
Jacoby et al. Enzyme Purification and Related Techniques, Academic Press, (1971), pp. 14-27.
Id. pp. 398-412.
Hill et al., Methods of Enzymology vol. XIX, "Proteolytic Enzymes", Academic Press, (1965), pp. 31-41.
Id., pp. 63-107.
Hill, Advances in Protein Chemistry 20: 37, 89-90 (Anifsen, Jr. et al., eds., 1965).
Sakina, et al., Int. J. Peptide Protein Res., 31: 245-52 (1988).
Kullman, Proc. Nat. Acad. Sci. (U.S.A.) 79: 2840-44 (1982).
Homandberg, et al., Biochemistry 21: 3385-89 (1982).
Chaiken, CRC Critical Reviews in Biochemistry, 255-301 (1981).
Ruggeri, et al., P.N.A.S. (U.S.A.) 83: 5708-12 (Aug. 1986).
Houghten et al., P.N.A.S. (U.S.A.) 82: 5131-35 (1985).
Goff et al., DNA 6: 381-88 (1987).
Jakubke et al., Angew Chem. Int. Ed. Engl. 24: 85-93 (1985).
Chaiken et al., Applied Biochem. Biotech. 7: 385-399 (1982).
Morihara et al., Trends, Biotech. 8: 164-170 (1987).
Fruton et al., (Journal not identified, 1950, pp. 39-53).
Whitaker and Bender, J. Am. Chem. Soc. 87: 2728-2737 (1965).
Fruton, "Enzymic Hydrolysis and Synthesis of Peptide Bonds", in Currents in Biochemical Research (D. E. Green, ed., 1946) pp. 123-135.
Fruton, Yale J. Biol. Med., 1950, pp. 263-271.
Kullmann, J. Biol. Chem. 255: 8234-8238 (1980).
Perham, R. N. Instrumentation in Amino Acid Sequence Analysis; Academic Press, NY, N.Y. .COPYRGT.1975.
Whitaker, John R. Principles of Enzymology for the Food Sciences; Mavcel Dekkev, Inc. NY, N.Y. .COPYRGT.1972.
Jakoby, W. Methods in Enzymology vol. XXII Academic Press, NY, N.N. .COPYRGT.1971.
Creighton Proteins W. H. Freeman Company (1984) pp. 39-42.
Hopfinger Anton J.
Le Breton Guy
Venton Duane L.
Cooper Iver P.
Green Lora M.
Kepplinger Esther M.
Receptor Laboratories, Inc.
LandOfFree
Method for generating and screening useful peptides does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for generating and screening useful peptides, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for generating and screening useful peptides will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1990794