Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1993-10-27
1997-07-01
Zitomer, Stephanie W.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435 6, 435 912, 435 691, 4351723, 435810, 436501, 536 231, 536 241, 536 243, 536 2431, 536 2432, 536 2433, 935 9, 935 77, 935 78, C12P 1934
Patent
active
056437617
ABSTRACT:
This invention provides a method of generating a subtracted cDNA library of a cell comprising: a) generating a cDNA library of the cell; b) isolating double-stranded DNAs from the cDNA library; c) releasing the double-stranded cDNA inserts from the double-stranded DNAs; d) denaturing the isolated double-stranded cDNA inserts; e) hybridizing the denatured double-stranded cDNA inserts with a labelled single-stranded nucleic acid molecules which are to be subtracted from the cDNA library; and f) separating the hybridized labeled single-stranded nucleic acid molecule from the double-stranded cDNA inserts, thereby generating a subtracted cDNA library of a cell.
REFERENCES:
Duguid, J.R., et al. (1988) Isolation of cDNAs of scrapie-modulated RNAs by subtractive hybridization of a cDNA library, Proc. Natl. Acad. Sci., USA, 85:5738-5742 (Exhibit B).
Hara, E., et al. (1991) Subtractive cDNA cloning using oligo(dT)-30-latex and PCR: Isolation of cDNA clones specific to undifferentiated human embryonal carcinoma cells, Nucleic Acids Research, 19(25):7097-7104.
Herfort, M.R., et al. (Nov. 1991) Simple and efficient subtractive hybridization screening, BioTechniques, 11(5):598-603 (Exhibit D).
Lee, S.W., et al. (Apr. 1991) Positive selection of candidate tumor-suppressor genes by subtractive hybridization, Proc. Natl. Acad. Sci, USA, 88:2825-2829 (Exhibit E).
Maniatis, T., et al. (1982) Strategies for cDNA cloning, Molecular Cloning, A Laboratory Manual, pp. 224-228 (Exhibit F).
Sive, H.L., et al. (Nov. 1988) A simple subtractive hybridization technique employing photoactivatable biotin and phenol extraction, Nucleic Acids Research, 16(22)10937 (Exhibit G).
Travis, G.H., et al. (Mar. 1988) Phenol emulsion-enhanced DNA driven subtractive cDNA cloning: Isolation of low-abundance monkey cortex-specific mRNAs, Proc. Natl. Acad. Sci. USA, 85:1696-1700 (Exhibit H).
Wieland, I., et al. (1990) A method for difference cloning, gene amplification following subtractive hybridization, Proc. Natl. Acad. Sci., USA, 87:2720-2724 (Exhibit I).
Audette et al. (1989) Molecular Immunology, vol. 26, No. 6, pp. 515-522.
Hara et al. (1993) Analytical Biochemistry, vol. 214, pp. 58-64.
Rubenstein et al. (1990) Nuc. Acid Res, vol. 18, No. 17, pp. 4833-4842.
Fisher Paul B.
Jiang Hongping
Chan Albert Wai-Kit
Marschel Ardin H.
The Trustees of Columbia University in the City of New York
White John P.
Zitomer Stephanie W.
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