Method for gene expression

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

Reexamination Certificate

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C536S023100

Reexamination Certificate

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10486416

ABSTRACT:
The invention relates to a method for gene expression in a cell-free transcription/translation system, the reaction solution containing all the components necessary for the transcription/translation mechanism, amino acids, nucleotides, metabolic components which provide energy and which are necessary for synthesis, and the proteins arising during translation being immobilized on a matrix.

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Merk, Helmut; Stiege, Wolfgang; Tsumoto, Kouhei; Kumagai, Izumi; Erdmann, Volker A. “Cell-Free expression of two single-chain monoclonal antibodies against lysozyme: effect of domain arrangement on the expression”. J. Biochem. vol. 125. 1999. pp. 328-333.
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